乙型肝炎病毒宫内感染新生儿外周血T细胞亚群表达  

Expression of peripheral blood T-lymphocyte Subsets in newborns with intrauterine infection of hepatitis B virus

作　　者：汪波[1] 高怡[2] 郭珍[3] 丰淑英 郭健[3] 张临瑞[3] 王婷[3] 王素萍[3] 

机构地区：[1]太原市第三人民医院妇产科,030012 [2]山西医科大学卫生毒理学教研室 [3]山西医科大学流行病学教研室

出　　处：《中国药物与临床》2014年第7期865-866,共2页Chinese Remedies & Clinics

基　　金：国家自然科学基金(81072341);山西省留学人员科研资助项目(2008-50);山西医科大学科技创新基金(C01201007)

摘　　要：目的探讨T细胞亚群在乙型肝炎病毒(HBV)宫内感染新生儿外周血中的表达和意义。方法选择HBV表面抗原(HBsAg)阳性孕妇及其分娩新生儿276例,用酶联免疫吸附试验检测孕妇和新生儿外周血HBV标志物,实时荧光定量聚合酶链反应(PCR)检测母亲和新生儿外周血HBV DNA含量,用流式细胞仪检测新生儿外周血CD3+、CD4+、CD8+T细胞亚群的水平。结果 HBV宫内感染的新生儿外周血CD3+、CD4+T细胞和CD4+/CD8+低于未感染新生儿,HBV宫内感染新生儿CD8+高于未感染新生儿,差异无统计学意义(P>0.05)。结论尚未发现HBV宫内感染新生儿外周血T细胞亚群表达失调。Objective To investigate the expression and significance of T-lymphocyte subsets in peripheral blood derived from newborns with intrauterine infection of hepatitis B virus （HBV）. Methods We recruited 276 HBV surface antigen （HBsAg） positive pregnant women and their newborns. The HBV markers were detected with enzyme-linked immunosorbent assay. HBV DNA contents were detected with real-time fluorescent quantitative PCR. The ex-pression of T-lymphocyte subsets （CD3＋, CD4＋and CD8＋） in peripheral blood derived from newborns were detected us-ing flow cytometry. Results The number of peripheral blood CD3＋and CD4＋cells and CD4＋/CD8＋ratio in newborns with intrauterine infection were significantly lower than those of non-intrauterine infection group （all P〈0.05）. Howev-er, the CD8＋cell count in newborns with intrauterine infection was numerically but not statistically greater than that in non-intrauterine infection group （P〉0.05）. Conclusion The current findings do not indicate imbalance of peripheral blood T-lymphocyte subsets in newborns with intrauterine infection of HBV.

关 键 词：肝炎 乙型 宫内感染 T淋巴细胞亚群 

分 类 号：R722.1[医药卫生—儿科]