hMDHⅡ过表达抑制过氧化氢压力下的CHO细胞凋亡  

Inhibition of Apoptosis under Hydrogen Peroxide Treatment through Over-Expression of Human Malate DehydrogenaseⅡ in Chinese Hamster Ovary Cells

作　　者：靖钰[1,2] 张存超[2] 付托[2] 蒋成[2] 张学光[1] 寇庚[2] 

机构地区：[1]苏州大学医学生物技术研究所,江苏苏州215007 [2]第二军医大学肿瘤研究所,上海200433

出　　处：《化学与生物工程》2014年第7期9-12,共4页Chemistry & Bioengineering

基　　金：国家863计划资助项目(2009A02Z107)

摘　　要：构建人苹果酸脱氢酶Ⅱ(hMDHⅡ)过表达的重组CHO细胞,并研究hMDHⅡ过表达对过氧化氢压力下CHO细胞凋亡的影响。结果显示,在0.1mmol·L-1过氧化氢压力下,hMDHⅡ过表达明显提高了补料分批培养后期细胞活率。线粒体膜电位及活性氧检测显示,hMDHⅡ过表达后线粒体膜电位显著提高、活性氧显著降低,表明hMDHⅡ过表达抑制细胞凋亡可能是通过减少细胞内活性氧而实现的。In the study,we constructed recombinant CHO cells over-expressing human malate dehydrogen-ase Ⅱ（hMDHⅡ）,and investigated its impact on apoptosis under hydrogen peroxide treatment.The results showed that hMDHⅡ over-expression improves the cell survival rate in fed-batch culture under 0.1 mmol·L-1 hydrogen peroxide treatment.Analysis on mitochondrial membrane potential and reactive oxygen species（ROS） showed that,after hMDHⅡ over-expression mitochondrial membrane potential increases significantly,which means reduction in apoptosis,and ROS decreases.This suggests that inhibition of apoptosis by over-expression of hMDHⅡ may due to decrease in ROS.This study will further the understanding on ROS-induced apoptosis, and also provide suggestions for the construction of cell line with the property of anti-apoptosis.

关 键 词：CHO细胞 人苹果酸脱氢酶Ⅱ 补料分批培养 凋亡 活性氧 

分 类 号：Q813.110[生物学—生物工程]