天麻素对神经病理性疼痛大鼠的镇痛作用及其机制研究  

Analgesic Effect and Mechanism of Gastrodin on the Neuropathic Pain in Rats

作　　者：徐敏[1] 刘勇军[2] 闵闽 陈洪涛[1] 徐莉[1] 顾华群[1] 

机构地区：[1]广州市第八人民医院麻醉科,广东广州510060 [2]中山大学第一附属医院普外科,广东广州510080 [3]广州市白云区石井人民医院普外科,广东广州510430

出　　处：《临床医学工程》2014年第7期837-839,共3页Clinical Medicine & Engineering

基　　金：广州市医药卫生科技项目(项目编号:20121A010005)

摘　　要：目的探讨天麻素对神经病理性疼痛大鼠的镇痛作用及其机制,为临床开发新的镇痛药物奠定基础。方法 SD大鼠随机分为坐骨神经慢性压迫性损伤组(n=32)和假手术组(n=8),自术后第8天分别腹腔注射天麻素治疗,用电子Von-Frey测痛仪测定大鼠机械缩足反射阈值,热痛刺激仪测定大鼠热痛缩足反应潜伏期。取脊髓腰膨大及L4/L6背根神经节,采用Western blotting法测定脊髓背角和背根神经节p-ERK1/2的表达。结果与假手术组相比,对照组和天麻素治疗组各时点机械缩足反射阈值降低、热痛缩足反应潜伏期缩短,脊髓背角及背根神经节p-ERK1/2均出现表达上调(P<0.05);与对照组相比,天麻素治疗组在给药后7 d机械缩足反射阈值回升、热痛缩足反应潜伏期延长;在给药14 d后脊髓背角及背根神经节p-ERK1/2表达下调(P<0.05)。结论天麻素可减轻大鼠神经病理性痛,其机制可能与抑制脊髓背角和背根神经节p-ERK1/2通路激活有关。Objective To investigate the analgesic effects and mechanism of gastrodin on the neuropathic pain in rats, and screen novel analgesics for clinical treatment of neuropathic pain diseases. Methods Forty male SD rats were randomly divided into 5 groups as sham operation group, control group, gastrodin 30 mg/kg group, gastrodin 60 mg/kg group, gastrodinl20 mg/kg group. From the 8~ day post-ligation, different doses of gastrodin were injected into abdominal cavity and the pain thresholds before and after intraperitoneal injection of the different doses of gastrodin were measured by the mechanical and hot stimulus. The expressions of p-ERK1/2 in LA/6 dorsal root ganglion neurons and dorsal horn was measured by the methods of Western blotting. Results Compared with sham operation group, the rats in CCI group developed a decrease in mechanical withdrawal threshold and thermal withdrawal latency associated with an increase in the activity of p-ERK1/2 in dorsal horn and dorsal root ganglion. Compared with CCI group, 7 day treatment with gastrodin significantly attenuated mechanical allodynia and thermal hyperalgesia, and these effects were correlated with inhibiting the hyper-activation ofp-ERK1/2 after 14 days treatment with gastrodin. Conclusions Gastrodin has beneficial effects on neuropathic pain and the analgesic effects of gastrodin would be mediated by the ERK1/2 signaling pathways.

关 键 词：天麻素 神经病理性疼痛 镇痛 p-ERKl 2通路 

分 类 号：R651.3[医药卫生—外科学]