三七总皂苷对β-淀粉样蛋白42损伤脑微血管内皮细胞ZO-1表达的影响  

Effects of Panax Notoginseng Saponins on Expression of ZO-1 of Brain Microvascular Endothelial Cells Caused by Aβ42

作　　者：杨丽鹍[1,2] 玛娜璐璐 朱海燕[2] 马涛[3] 孙逸坤[2] 赵一舟[2] 高永红[2] 

机构地区：[1]北京市中医药大学东直门医院东区,北京101100 [2]北京中医药大学东直门医院,中医内科学教育部重点实验室和北京市重点实验室,北京100700 [3]北京中医药大学东方医院,北京100078

出　　处：《中国中医药信息杂志》2014年第8期57-59,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81173229;81202686);北京中医药大学实验室开放课题(2011-SYSKFKT-01-2)

摘　　要：目的观察三七总皂苷对β-淀粉样蛋白(Aβ)42损伤脑微血管内皮细胞ZO-1表达的影响,为阿尔茨海默病的对因治疗提供新的依据。方法细胞分为正常对照组、模型组和三七总皂苷低、中、高剂量组,37℃、5%CO2培养箱中培养24 h后,采用MTT比色法检测细胞活力,Western blot检测ZO-1蛋白表达。结果 Aβ42刺激后微血管内皮细胞活力降低(P<0.01),ZO-1蛋白表达被抑制(P<0.01);与模型组比较,三七总皂苷低、中、高剂量组微血管内皮细胞活力增加,其中三七总皂苷中、高剂量组增加较为明显,差异有统计学意义(P<0.05),ZO-1蛋白表达增加。结论三七总皂苷通过抑制Aβ42所致微血管内皮细胞活力降低,恢复血脑屏障的部分屏障功能。Objective To study the effects of Panax Notoginseng Saponins （PNS） on the damaged expression of ZO-1 of brain microvascular endothelial cells caused by Aβ42.MethodsBrain microvascular endothelial cells were divided into normal control group, model group, PNS low-, medium- and high concentration groups. They were incubated for 24h in 5% CO2 incubator at 37℃ . Then cell vitality was detected by MTT colorimetric method and ZO-1 protein expression was tested by Western blot.Results Stimulation of Aβ42 reduced the activity of microvascular endothelial cells （P〈0.01） and suppressed the expression of ZO-1 protein （P〈0.01）. Compared with the model group, the activity of microvascular endothelial cells of PNS groups, especially the high and medium dose groups （P〈0.05）, and increased the ZO-1 protein expression. Conclusion PNS can partly recover the barrier function of blood brain barrier through inhibiting the decrease of the activity of microvascular endothelial cells caused by Aβ42.

关 键 词：三七总皂苷 阿尔茨海默病 β-淀粉样蛋白42 ZO-1蛋白 

分 类 号：R285.5[医药卫生—中药学]