升清中药对饮食诱导肥胖大鼠瘦素抵抗的影响  

Effect of Shengqing Herbs on Leptin Resistance of Diet-Induced Obesity Rats

作　　者：姜萍[1] 宋钦兰[2] 

机构地区：[1]山东中医药大学,山东济南250355 [2]山东中医药大学附属医院,山东济南250011

出　　处：《中国中医药信息杂志》2014年第8期60-63,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81102676)

摘　　要：目的探讨升清中药抑制饮食诱导肥胖(DIO)大鼠肥胖及瘦素抵抗的作用机制。方法取Wistar大鼠,制作DIO大鼠40只和DIO抵抗(DIO-R)大鼠10只。DIO大鼠分为模型组、西布曲明组、健脾理气组、升清组,每日分别以生理盐水、西布曲明、健脾理气药物(陈皮、木香、枳实)、升清药物(柴胡、升麻、葛根)灌胃,空白组与DIO-R组予生理盐水,灌胃16周。测定干预前后体质量,检测干预后血清神经肽Y(NPY)、瘦素和脂肪瘦素、细胞因子信号传导抑制因子3(SOCS-3)。结果与DIO-R组比较,模型组体质量增加、NPY升高(P<0.01),血清和脂肪瘦素降低(P<0.05),SOCS-3升高(P<0.05)。药物干预后,各药物组体质量有所下降。健脾理气组脂肪瘦素升高(P<0.05);升清组在升高瘦素、降低体质量和NPY、SOCS-3水平方面,优于西布曲明组和健脾理气组,差异有统计学意义(P<0.05,P<0.01)。结论升清药物能减轻肥胖,抑制瘦素抵抗。Objective To observe the effects of Shengqing Chinese herbs on the obesity and leptin resistance of diet-induced obesity （DIO） rats.Methods Wistar rats were produced a rat model including of 40 DIO rats and 10 DIO-R rats. The DIO rats were divided into DIO model group, sibutramine group, Jianpi Liqi group and Shengqing group, which were respectively by gavage of saline, sibutramine, Jianpi Liqi herbs （Citri reticulatae pericarpium, Aucklandiae radix, Aurantii fructus immaturus）, Shengqing herbs （Bupleuri radix, Cimicifugae rhizoma, Puerariae lobatae radix）, blank control group and DIO-R group were by gavage of saline. Their weight, were examined after 16 weeks gavage. Leptin and neuropeptide Y in the serum were examined. Leptin, TNF-α, and suppressors of cytokine signaling-3 （SOCS-3） in the homogenized fat were examined. Results Compared with the DIO-R group, DIO model group＇s body weight, NPY significantly increased （P〈0.01）;leptin in the serum and homogenized fat decreased （P〈0.05）;SOCS-3 increased （P〈0.05）. After drug intervention, weight of each drug group declined. Compared with the DIO model group, Jianpi Liqi group’s leptin in the homogenized fat increased （P〈0.05）. Shengqing group＇s leptin in homogenized fat increased, body weight reduced, NPY and SOCS-3 levels decreased, and better than sibutramine group and Jianpi Liqi group （P〈0.05,P〈0.01）.Conclusion Shengqing herbs can reduce obesity and inhibit leptin resistance.

关 键 词：肥胖 瘦素 瘦素抵抗 神经肽Y 细胞因子转录负调节因子 升清药物 健脾理气药物 大鼠 

分 类 号：R285.5[医药卫生—中药学]