银杏叶提取工艺正交试验研究  

Orthogonal Experiment Study on Extraction Process of Ginkgo Leaves

作　　者：姚建标 王建方 吴旭明 金辉辉 陈仲良[2] 

机构地区：[1]浙江现代中药与天然药物研究院有限公司,浙江杭州310052 [2]中国科学院上海药物研究所,上海200031

出　　处：《中国中医药信息杂志》2014年第8期78-80,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：浙江省科技计划项目(2009E10009);浙江省企业研究院建设计划(2010E90011)

摘　　要：目的以总黄酮醇苷、萜类内酯的综合转移率为指标,确定银杏叶最佳提取工艺。方法采用正交设计方法,系统考察乙醇浓度、料液比、提取时间3个因素对银杏叶提取工艺的影响,利用高效液相色谱法测定总黄酮醇苷、萜类内酯的含量,计算转移率。结果最佳提取工艺条件为:采用85%乙醇加热回流提取3次,第1次加5倍量(V/W)溶剂提取3 h,后2次分别加入3倍量(V/W)溶剂提取2 h。结论本工艺操作简单、合理、节能、高效,适用于工业化生产。Objective To determine the best extraction process of ginkgo leaves with the total transfer rate of total flavonol glycosides and terpene lactones as the index.Methods The effects of ethanol concentration, solid-liquid ratio and extraction time on extraction process were investigated by orthogonal design method, and the contents of total flavonol glycosides and terpene lactones were detected by HPLC to calculate transfer rate.Results The optimum extraction conditions were as follows：85% ethanol refluxing and extracting for three times;the first time extracting with five-fold amount of solvent （V/W） for 3 hours;the last two times with three-fold solvent （V/W） for 2 hours.Conclusion This extraction process has the advantages of simplicity of operator, reason, energy conservation, high efficiency, and is suitable for industrial production.

关 键 词：银杏叶 总黄酮醇苷 萜类内酯 转移率 正交试验 

分 类 号：R283.5[医药卫生—中药学]