不同强度运动对青春期肥胖大鼠血糖、胰岛素敏感及RBP4的影响  

Effects of different intensity exercise on blood glucose,adolescent obesity rats insulin sensitivity and RBP4

作　　者：崔建飞[1] 柏友萍[1] 李萌[1] 许晓东[1] 戴圆圆[1] 张晶[1] 

机构地区：[1]安徽师范大学体育学院,芜湖241003

出　　处：《卫生研究》2014年第4期535-540,共6页Journal of Hygiene Research

基　　金：安徽省高校自然科学基金重点项目(No.KJ2013A138)

摘　　要：目的探讨不同强度运动对青春期肥胖大鼠血糖、胰岛素敏感及RBP4的影响。方法110只鼠龄21天SD幼鼠建模7周后，取32只肥胖倾向大鼠随机分为4组，每组8只，分别为对照组和低、中、高强度运动组（动物跑台跑速分别定为15～18、21-25和28-32m／min，5次／周，60min／次，连续8周）。运动干预后麻醉采集血液及内脏组织，测血糖、血胰岛素，测血RBP4浓度、肝脏组织RBP4蛋白表达及内脏脂肪组织RBP4mRNA表达。结果8周运动干预后，低、中、高强度运动组血糖明显低于对照组，胰岛素敏感性高于对照组（P〈0．01），胰岛素低于对照组但没有差异。低、中、高强度运动组内脏脂肪组织RBP4mRNA表达低于对照组，表达显著下调（P〈0．01），中、高强度组较低强度组下调明显（P〈0．01）。肝脏RBP4蛋白表达为对照组高于运动组，中、高强度运动组表达较少；血浆RBP4浓度为低、中、高强度运动组明显低于对照组（P〈0．01），高强度运动组低于中、低强度组（P〈0．01）。结论通过不同强度运动干预能降低青春期肥胖大鼠血糖和血RBP4浓度，提高胰岛素敏感性，下调内脏脂肪组织RBP4mRNA表达水平，肝脏组织RBP4蛋白表达下降，随着运动强度增加效果明显。Abstract：Objective To discuss the effects of different intensity exercise on blood glucose, adolescent obesity rats insulin sensitivity and RBP4. Methods The model of rats after 7 weeks, 32 rabbits of obesity prone rats were randomly divided into 4 groups, 8 rats in each group, respectively, control group, low intensity exercise group, moderate intensity exercise group, high intensity exercise group. High strength low in animal treadmill running speed respectively for 15 - 18, 21 -25 and 28 -32m/min, 5 times / week, 60min/ times, for 8 consecutive weeks. Anesthesia for blood and tissue collection after exercise intervention, blood glucose, blood insulin, blood RBP4 concentration, liver RBP4protein expression and visceral adipose tissueRBP4 mRNA expression. Result The 8 week exercise intervention, low, high strength, in the sugar group was significantly lower than the control group （P 〈 0.01）. Insulin sensitivity was higher than that of the control group （P 〈0.01 ） , insulin was lower than the control group difference. Expression of visceral adipose tissue RBP4 mRNA was low, medium and high strength group was lower than the control group, the expression was significantly down regulated （P 〈 0.01 ） , in the high intensity group, low intensity group lowered significantly （P 〈 0.01 ）. RBP4 higher than exercise group as control group in the expression of liver tissue protein, in the high intensity group, less the amount of expression. Plasma RBP4 concentration of high strength group was significantly lower than that of the control group （P 〈 0.01 ） , high strength, low intensity group were lower than those in group （P 〈 0.01 ）. Conclusion Through the different intensity exercise intervention can reduce blood glucose in obese rats, the plasma RBP4 concentration, improve insulin sensitivity, lower expression of RBP4 mRNA in adipose tissues, liver protein expression decreased, with the exercise intensity increased significantly.

关 键 词：运动强度 青春期肥胖脂肪组织 肝脏组织 胰岛素敏感 RBP4 

分 类 号：R179[医药卫生—妇幼卫生保健] R195.2[医药卫生—公共卫生与预防医学]