祛瘀化痰软肝丸对实验性肝硬化大鼠模型干预机理的实验研究  

Experimental study of Quyu Huatan Ruangan Pill on mechanism intervention in experimental cirrhotic rats

作　　者：孙林 

机构地区：[1]甘肃省庆阳市中医医院,甘肃庆阳45000

出　　处：《中医临床研究》2014年第15期40-43,共4页Clinical Journal Of Chinese Medicine

基　　金：2012年甘肃省庆阳市科技局科研支撑项目:"祛瘀(化痰)软肝丸治疗肝硬化的机理研究和临床观察"项目编号:KZ2012-40

摘　　要：目的:研究"祛瘀化痰软肝丸"对实验性肝硬化大鼠模型的作用机理。方法:Wistar雄性大鼠60只随机分为5组,每组12只。除正常组外,模型组、西药组和中药组均给予大鼠皮下注射40%四氯化碳(CCl4)花生油溶液2.5 ml/kg,每周注射2次,连续注射11周。连续注射CCl4的最后2周(即已注射9周后),中药组同时用祛瘀化痰软肝丸灌胃治疗;西药组以秋水仙碱灌胃治疗;正常组和模型组则给予等体积生理盐水灌胃;各组连续干预2周后,检测各组大鼠肝功能。结果:与模型组比较,祛瘀化痰软肝丸组血清总蛋白(TP)、血清清蛋白(ALB)含量明显升高,丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)含量明显下降,差异均有统计学意义(P<0.01或P<0.05);肝纤维化四项指标:肝组织透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(ⅣC)含量均明显下降,差异均有统计学意义(P<0.01或P<0.05);结论:祛瘀化痰软肝丸可显著提高TP、ALB含量,降低ALT、AST、HA、LN、PCⅢ及ⅣC含量。祛瘀化痰软肝丸在一定程度上能有效地保护肝细胞,恢复肝功能,降低肝纤维化四项指标,具有较好的防治实验性大鼠肝硬化模型肝功能和肝纤维化的作用。Objective：To study the mechanism of Quyu Huatan Ruangan Pill in experimental cirrhotic rats. Methods：60 male Wistar rats were randomly divided into 5 groups, 12 rats in each group. Except the normal group, the rats in the model group, western medicine group and Chinese medicine group were given subcutaneous injection of 40%carbon tetrachloride （CCl4） of peanut oil solution for 2.5 ml/kg, 2 times per week, 11 weeks of continuous injection. During the last 2 weeks of injection of CCl4 （after injection for 9 weeks）, rats in the Chinese medicine group were also treated with Quyu Huatan Ruangan Pill by gavage;rats in the western medicine group treated with colchicine by gavage;while rats in the normal group and model group were given equal volume of normal saline;after 2 weeks, the liver function of rats in each group were detected. Results：compared with the model group, the total serum protein（TP）,serum albumin （ALB） of rats in the Quyu Huatan Ruangan Pill group increased obviously, alanine aminotransferase （ALT）, aspartate aminotransferase （AST） levels decreased significantly and the differences were statistically significant （P〈0.01 or P〈0.05）;four indicators of hepatic fibrosis：hyaluronic acid （HA）, laminin （LN）, procollagen type Ⅲ （PC Ⅲ）, collagen type IV （C IV） obviously decreased and the differences were statistically significant （P〈0.01 or P〈0.05）. Conclusion： Quyu Huatan Ruangan Pill can significantly improve the content of TP, ALB, reduce the content of ALT, AST, HA, LN, PC Ⅲ and ⅣC. To a certain extent, Quyu Huatan Ruangan Pill can effectively protect the liver cells, help recover the liver function, reduce the four indicators of hepatic fibrosis with good prevention and treatment of liver function damage and liver fibrosis in experimental liver cirrhosis model rats.

关 键 词：祛瘀化痰软肝丸 肝硬化 大鼠模型 实验研究 

分 类 号：R96[医药卫生—药理学]