Pharmacological Evaluation of Hexane Fraction of Casearia gossypiosperma Briquet： Antivenom Potentiality  

作　　者：Juliana de Oliveira Soares-Silva Jhones Luiz de Oliveira José Carlos Cogo Renata Vasques da Silva Tavares Yoko Oshima-Franco 

机构地区：[1]University of Sorocaba (UNISO), Sorocaba 18023-000, SP, Brazil [2]Serpentarium of the Vale do Paraiba University, CEN, UNIVAP, Sao José dos Campos 12244-000, SP, Brazil

出　　处：《Journal of Life Sciences》2014年第4期306-315,共10页生命科学（英文版）

摘　　要：Neuromuscular junction is the main target for snakebites, which venoms act depending on the snake genus for paralyzing the prey （neurotoxicity） or for facilitating the digestion processes of the victim （myotoxicity）, and also as a defense mechanism against predators. In the present study, a mouse neuromuscular apparatus was used for testing the hexane fraction of Casearia gossypiosperma plant, that showed the better antiophidian ability than dichloromethane, ethyl acetate and methanol fractions, against the irreversible paralysis induced by two, Bothrops jararacussu （Bjssu, 40 μg/mL） and Crotalus durissus terrificus （Cdt, 10 μg/mL） snake venoms. All fractions were obtained by liquid-liquid partition from the C. gossypiosperma hydroalcoholic lyophilized extract. The preliminary chromatographic profile of this plant showed phenols and flavonols as active constituents, whereas hexane fraction expressed mainly 13-sitosterol and quercetin. In spite of hexane fraction protection （≌95% and 48% against Bjssu and Cdt, respectively）, isolately, only quercetin protected against the blockade-induced by Bjssu venom （65.5%）. This study showed that hexane fraction acts against these snake venoms by a synergistic phytocomplex mechanism.

关 键 词：Bothrops jararacussu Casearia gossypiosperma Briquet Crotalus durissus terrificus neuromuscular junction pharmacological evaluation. 

分 类 号：TQ460[化学工程—制药化工] O623.11[理学—有机化学]