Expression Variability of Carbohydrates at the Cell Wall Surface of Aspergillus Species and in Vitro Susceptibility to Amphotericin B, Voriconazole and Itraconazole  

作　　者：André Ferraz Goiana Leal Eduardo Isidoro Cameiro Beltrao Almir Goncalves Wanderley Rejane Pereira Neves 

机构地区：[1]Department of Mycology, Federal University of Pernambuco, Recife, PE, Brazil [2]Department of Biochemistry, Federal University of Pernambuco, Recife, PE, Brazil [3]Keizo Asami Immunopathology Laboratory (LIKA), Federal University of Pernambuco, Recife, PE, Brazil [4]Department of Physiology and Pharmacology, Federal University of Pernambuco, Recife, PE, Brazil

出　　处：《Journal of Life Sciences》2014年第4期358-363,共6页生命科学（英文版）

摘　　要：This study evaluated the expression of N-acetyl-D-glucosamine, L-fucose, D-galactose and glucose/mannose on the cell wall surface of Aspergillus species using lectins and the in vitro antifungal activity of AMB （amphotericin B）, VOR （voriconazole） and ITC （itraconazole）. Con A （Concanavalin A）, WGA （wheat germ agglutinin）, UEA-I （Ulex europeus agglutinin I） and PNA （peanut agglutinin）, all conjugated with horseradish peroxidase, were used. For the susceptibility tests, sterilized fiat-bottomed 96-well microtitre plates were used and the procedures were followed of the Clinical and Laboratory Standard Institute. The inoculum was added to the wells with the tested drugs and the plates were incubated at 25 ℃ for 48 h before reading the results to determine the MIC （minimal inhibitory concentration） of AMB, VOR and ITC. All Aspergillus strains showed low MIC for AMB （0.031-1 μg/mL） and ITC （0.031-0.25μg/mL）. However, the isolates were less susceptible to VOR, for which the MIC ranged from 4 to 16 μg/mL. The results of this study indicate that the expression of N-acetyl-D-glucosamine and glucose/mannose on the cell wall surface of the Aspergillus species evaluated showed greater stability of expression in the fungal growth both in vitro and in parasitism.

关 键 词：Cell wall carbohydrates LECTINS ASPERGILLUS antifungal activity. 

分 类 号：S858.33[农业科学—临床兽医学] TQ465.7[农业科学—兽医学]