活血解毒方干预NOD小鼠自发性干燥综合征的机制研究  

Mechanism study of Huoxue Jiedu Formula on spontaneous Sjogren's syndrome in NOD mice

作　　者：钱荔[1] 侯秀娟[1] 刘小平[1] 袁芳[1] 史云晖 朱跃兰[1] 

机构地区：[1]北京中医药大学东方医院,北京100078

出　　处：《北京中医药》2014年第6期467-469,共3页Beijing Journal of Traditional Chinese Medicine

基　　金：北京中医药大学研究生自主课题(2013-JYBZZ-XS-147)

摘　　要：目的探讨活血解毒方对非肥胖糖尿病(NOD)白发性干燥综合征(SS)小鼠的疗效及作用机制。方法将18只NOD/Ltj小鼠随机分为模型组、活血解毒方组、白芍总苷组各6只,并设6只C57BL/6J小鼠作为正常组,灌胃8周后取小鼠颌下腺及血清分别采用反转录-聚合酶链反应(RT-PCR)法和ELISA法检测Toll样受体TLR4、CD14的mRNA和蛋白表达。结果与正常组比较,模型组TLB4、CD14的mRNA和蛋白表达明显升高,差异有统计学意义(P<0.05);与模型组比较,白芍总苷组、活血解毒方组TLR4、CD14的mRNA和蛋白表达水平均有降低,差异有统计学意义(P<0.05);白芍总苷组、活血解毒方组2组间比较,TLR4、CD14的mRNA和蛋白表达差异均无统计学意义(P>0.05)。结论活血解毒中药对NOD小鼠SS具有较好的治疗作用,其作用机制可能与TLR4/CD14信号转导通路有关。Objective To investigate the efficacy and mechanism of action of Huoxue Jiedu Formula （HXJD） on spontaneous Sjogren＇ s syndrome （SS） in non-obese diabetic （NOD） mice. Methods： Eighteen NOD/Ltj mice were randomly divided into a model group, HXJD group and a total glucosides of paeony （TGP） group, 6 cases in each one, and 6 C57BL/6j mice were set as the normal group. The mouse submandibular gland and serum were get to detect the expression of toll-like receptor TLR4, CD14mRNA and protein by reverse transcription polymerase chain reaction （RT-PCR） method and ELISA method respectively after 8 weeks of intragastric administration. Results Compared with the normal group, the expression of TLR4 and CD14 mRNA and protein in the model group was increased significantly （P 〈 0. 05 ） ; Compared with the model group, the expression of TLR4 and CD14 mRNA and protein in the TGP and HXJD group was significantly decreased （ P 〈 0. 05 ） ; The expression of TLR4 and CD14 mRNA and protein between the TGP group and HXJD group had no significant difference （P 〉 0. 05）. Conclusion HXJD has a better effect on SS for the NOD mice, and its mechanism might be related to the TLR4/CD14 signal transduction pathway.

关 键 词：干燥综合征 活血解毒方 信号转导通路 非肥胖糖尿病小鼠 实验研究 

分 类 号：R285.5[医药卫生—中药学]