Cr(Ⅵ)对果蝇S2细胞增殖及氧化应激的影响  

Research of drosophila S2 cell proliferation and oxidative stress injury induced by Cr(Ⅵ)

作　　者：夏嫱[1] 朱伟[1] 吴启仙 方旭[1] 杜联峰[1] 

机构地区：[1]遵义医学院珠海校区免疫学与病原生物学教研室,贵州省免疫学研究生教育创新基地,广东珠海519041

出　　处：《现代预防医学》2014年第15期2793-2795,共3页Modern Preventive Medicine

基　　金：国家自然科学基金(31260528);贵州省卫生厅优秀医学青年科技人才基金(gzwkj2010-2-003)

摘　　要：目的研究Cr(VI)对果蝇S2细胞增殖及氧化应激相关指标的影响,初步探讨二者的关系,为利用果蝇S2细胞研究Cr(VI)的毒性作用提供一定的理论依据。方法利用含不同浓度Cr(VI)(50、100、200、400、800μmol/L)的培养基培养果蝇S2细胞;CCK-8法检测培养基中不同浓度Cr(VI)对果蝇S2细胞增殖的影响;分光光度法检测培养基上清中SOD、MDA含量。结果随着Cr(VI)浓度升高,S2细胞的生存率逐渐降低并存在显著的剂量依赖关系,当800μmol/L时Cr(VI)对S2细胞的抑制率已高达(88.51±0.72)%;SOD活力自200μmol/L处理浓度开始即与对照组相比差异有统计学意义(P<0.05),随Cr(VI)处理浓度增加SOD含量逐渐降低;各处理组脂质氧化终产物MDA含量与对照相比差异均有统计学意义(P<0.05),随Cr(VI)处理浓度增加MDA含量逐渐升高。结论 Cr(VI)可显著抑制S2细胞增殖,且呈高度剂量依赖性;Cr(VI)可导致S2细胞氧化应激,氧化应激可能是Cr(VI)抑制S2细胞增殖的重要原因之一。Objective To investigate the effect of Cr （Ⅵ） on Drosophila S2 cell proliferation and cellular oxidative stress. Methods Drosophila S2 cells were cultured by medium containing different concentrations of Cr （Ⅵ） （50, 100, 200, 400, 800μmol/L）. CCK8 test was used to evaluate the suppression rate. Spectrophotometer was used to detect SOD, and MDA contents in supernatant of the medium. Results With the elevated Cr （Ⅵ） concentration, S2 cells survival rate decreased, and there was a dose-dependent relationship.When Cr （Ⅵ） concentration reached 800μmol/L, the S2 cell inhibition rate was up to 88.51 ± 0.72%. With the elevated Cr （Ⅵ） concentration, the SOD content decreased gradually. When Cr （Ⅵ） concentration reached 200μmol/L and more, the SOD activity had significant difference compared with the control group （P〈0.05）. MDA content in each treatment group had significant difference compared with the control （P〈0.05）. With the elevated Cr （Ⅵ） concentration, the MDA content increased gradually. Conclusion Cr （Ⅵ） can inhibit the proliferation of S2 cells and shows a high degree of dose-dependence; Cr （Ⅵ） leads to cellular oxidative stress, and cellular oxidative stress may be one of the reasons of inhibiting cell proliferation.

关 键 词：六价铬 果蝇S2细胞 细胞增殖 氧化应激 

分 类 号：R115[医药卫生—公共卫生与预防医学]