石菖蒲挥发油改善氯化锂-匹鲁卡品致痫大鼠行为学的抗氧化机制探讨  

Antioxidative mechanism of Acorus tatarinowii Schott volatile oil for improving the behavioral improvement of epilepsy rats induced by lithium-pilocarpine

作　　者：龚磊[1] 许洁[2] 龚其海[3] 蒋智刚[2] 肖庆邦[4] 周旭美[3] 刘露[3] 罗丽[3] 范瑞明[5] 

机构地区：[1]遵义医学院附属医院药剂科,贵州遵义563003 [2]遵义医学院公共卫生学院预防医学教研室,贵州遵义563003 [3]遵义医学院药学院,贵州遵义563003 [4]遵义医学院附属医院病理科,贵州遵义563003 [5]遵义医学院附属医院神经内科,贵州遵义563003

出　　处：《现代医药卫生》2014年第14期2081-2082,2085,共3页Journal of Modern Medicine & Health

基　　金：教育部长江学者和创新团队发展计划项目(IRT1197);贵州省中医药管理局课题(黔中医药发[2011]78号);遵义市红花岗区科技局基金课题[遵红科社字(2012)12号]

摘　　要：目的探讨石菖蒲挥发油对氯化锂-匹鲁卡品致痫大鼠行为学改善的抗氧化机制。方法将成年雄性SD大鼠66只随机分为对照组(6只)、癫痫组(30只)、石菖蒲挥发油组(30只),后两组采用氯化锂-匹鲁卡品法建立癫痫动物模型;观察各组行为学变化。癫痫模型大鼠在致痫后4、24、48 h断头取脑,检测海马组织中丙二醛(MDA)、一氧化氮(NO)、超氧化物歧化酶(SOD)的含量。结果 (1)行为学:癫痫组与石菖蒲挥发油组首达4级发作时间潜伏期比较,差异有统计学意义(P<0.05)。(2)生化指标改变:癫痫组较石菖蒲挥发油组MDA、NO含量明显升高、SOD含量明显下降,差异均有统计学意义(P<0.05),癫痫组和石菖蒲挥发油组的自由基变化与对照组比较,差异均有统计学意义(P<0.05)。结论石菖蒲挥发油能改善氯化锂-匹鲁卡品致痫大鼠的行为,并可能通过抑制MDA、NO含量的增加和SOD的减少,保护海马区神经元损伤。Objective To investigate the antioxidative mechanism of Acorus tatarinowii Schott volatile oil on the behav-ioral improvement of epilepsy rats induced by lithium-pilocarpine. Methods Sixty-six adult male SD rats were randomly divided into control group （n=6）,epilepsy group （n=30）,volatile oil of Acorus tatarinowii Schott group （n=30）,and the rat models of the latter two groups were established with lithium-pilocarpine method;the behavioral changes of all the groups were observed. The rats in the epilepsy group were decapitated at the 4th,24th and 48th h after epilepsy,to detect the content of methane dicarboxylic aldehyde （MAD）,nitric oxide （NO） and superoxide dismutase （SOD） in hippocampus. Results （1）The behavioristics showed that the comparison of incubation period between the epilepsy group and volatile oil of Acorus tatarinowii Schott group reached to level 4 had statistically significant difference（P〈0.05）. （2）The changes of biochemical indexes manifested that the contents of MDA and NO in the epilepsy group and volatile oil of Acorus tatarinowii Schott group increased obviously ,while the contents of SOD decreased prominently,and the differences between the two groups had statistical significance （P〈0.05）;compared with the control group,the free radical changes in the epilepsy group and volatile oil of Acorus tatarinowii Schott group had statistically significant difference（P〈0.05）. Conclusion The volatile oil of Acorus tatarinowii Schott can improve the behavior of epilepsy rates induced by lithium-Pilocorpine and inhibit the content of MDA,NO and SOD,so it is involved in the protection of neuronal damage of hippocampus.

关 键 词：石菖蒲 化学 油类 挥发 疾病模型 动物 癫痫 超氧化物类 

分 类 号：R969.1[医药卫生—药理学]