低剂量硝酸镧对糖尿病大鼠肝脏PPAR-γ和GLUT_4表达的影响  

Effect of the low doses of lanthanum nitrate in diabetic rat liver PPAR-γ and GLUT_4 expression

作　　者：李冬梅[1] 史勇[1] 黄可欣[2] 马洪喜[3] 

机构地区：[1]长春大学特殊教育学院,吉林长春130022 [2]吉林大学基础医学院 [3]吉林大学第一医院病理科

出　　处：《毒理学杂志》2014年第3期173-175,共3页Journal of Toxicology

基　　金：国家自然科学基金(29890280-3(2))

摘　　要：目的探讨口服低剂量硝酸镧对糖尿病大鼠肝脏PPAR-γ和GLUT4表达的影响。方法以Wistar雄性大鼠为研究对象,将实验动物分为3组即对照组(10只)、糖尿病模型组(18只)和硝酸镧给药组(12只),3组大鼠分别每日给予生理盐水、生理盐水、硝酸镧(0.2 mg/kg)灌胃1个月,然后处死取血清和肝脏。采用ELISA法检测大鼠血清中PPAR-γ和GLUT4的蛋白含量;采用免疫组织化学方法检测大鼠肝脏PPAR-γ和GLUT4的阳性表达强度;采用苏木素-伊红(HE)染色检测肝脏组织的病理改变。结果与对照组比较,硝酸镧给药组和糖尿病模型组,两组大鼠血清中PPAR-γ和GLUT4的蛋白含量及肝脏组织中PPAR-γ和GLUT4的蛋白阳性表达均降低差异有统计学意义(P<0.01);与硝酸镧给药组比较,糖尿病模型组更为显著(P<0.001)。结论口服低剂量硝酸镧(0.2 mg/kg)可通过上调大鼠血清及肝脏PPAR-γ蛋白和GLUT4蛋白,从而对肝组织有一定的保护作用。Objective To investigate the effects of the low-dose oral administration of lanthanum nitrate in diabetic rat liver PPAR-γ and GLUT4expression.Methods In Wistar male rats for the study,the experimental animal were divided into three groups： the control group（ 10）,diabetic model group（ 18） and lanthanum nitrate administration group（ 12）,three groups of rats given daily saline,saline,lanthanum nitrate（ 0.2 mg /kg） administered a month,and then sacrificed for the collection of the serum and liver samples.By ELISA to detect the protein content of PPAR-γ and GLUT4of the serum; Immunhistochemistry was used to detect the intensity of PPAR-γ and GLUT4expression; HE staining to detect pathological changes in liver tissue.Results Compared with the control group,lanthanum nitrate administration group and diabetic model group,two rats serum PPAR-γ and GLUT4protein content and protein expression of PPAR-γ and GLUT4in liver tissue were decreased significantly（ P ＆lt; 0.01）; Compared with lanthanum nitrate administration group,diabetes model group more significant（ P ＆lt; 0.001）.Conclusion Low-dose oral lanthanum nitrate（ 0.2 mg /kg） can increase serum and liver PPAR-γ and GLUT4protein in rat,and in order to the liver has a protective effect.

关 键 词：硝酸镧 糖尿病 肝脏 过氧化物酶体增殖物激活受体-Γ 葡萄糖转运蛋白4 

分 类 号：R943.5[医药卫生—药剂学]