铬离子对成骨细胞的毒性及对Tnfrsf17基因的影响  

Effects of chromium ions on cytotoxicity and Tnfrsf 17 of osteoblast

作　　者：高金巍[1] 李大河[1] 杨生生[1] 缪明永[1] 徐卫东[1] 

机构地区：[1]第二军医大学长海医院,上海200433

出　　处：《毒理学杂志》2014年第3期199-203,共5页Journal of Toxicology

基　　金：国家自然科学基金项目(81171686)

摘　　要：目的观察Cr3+对成骨细胞SaO2的细胞毒性以及在Cr3+的作用下对成骨细胞Tnfrsf17基因的表达的影响。方法体外培养SaO2成骨细胞,MTT法检测细胞活力,ALP试剂盒检测成骨细胞碱性磷酸酶的分泌情况,Von Kossa试剂盒检测细胞钙结节形成。通过Rt-PCR及Western检测Cr3+对Tnfrsf17表达的影响。结果在Cr3+的刺激作用下,与对照组相比,成骨细胞在相应时间节点细胞活性和钙结节形成明显受限,ALP的分泌呈时间与剂量依赖性的降低。在Cr3+的刺激作用下,0.1、1.3和150 mg各干预组与对照组相比,Tnfrsf17的基因表达水平及蛋白表达水平在24、48和72 h后均有所下降。结论 Cr3+对成骨细胞有细胞毒性而且可以下调Tnfrsf17基因及其蛋白产物的表达。Objective To study the effects of chromium ions on cytotoxicity and Tnfrsf17 of osteoblast SaO2.Methods Osteoblast SaO2was cultured in vitro.Cell viability was detected by MTT.Secretion of alkaline phosphatase and calcium nodules were detected by ALP kit and Von Kossa kit respectively.Effects of Chromium Ions on Tnfrsf17 of osteoblast was detected by Rt-PCR and Western.Results Compared with the control group,cytoactive and the formation of calcium nodules of osteoblast was distinctly impaired under the stimulation of Cr3 ＋,while the secretion of ALP was reduced with time and dosage.Compared with the control group by 0.1 mg,1.3 mg,150 mg group,the expression of Tnfrsf17 gene and its protein product declined after 24 h,48 h,and 72 h respectively.Conclusions Cr3 ＋has cytotoxicity on osteoblasts and can down-regulate the expression of Tnfrsf17 gene and its protein production.

关 键 词：成骨细胞 铬离子 Tnfrsf17 

分 类 号：R318[医药卫生—生物医学工程]