免疫磁珠筛选大鼠牙髓干细胞、外胚间充质干细胞的表型研究  

Phenotypic study of rat dental stem pulp cell and ectomesenchymal stem cell screened by immunomagnetic bead

作　　者：王亦菁[1] 张晓东[1] 孙淏海[1] 金岩[2] 史俊南[3] 

机构地区：[1]沈阳军区总医院口腔内科,辽宁沈阳110840 [2]第四军医大学口腔医学院组织工程中心,陕西西安710032 [3]第四军医大学口腔医学院牙体牙髓科,陕西西安710032

出　　处：《口腔医学》2014年第6期454-456,480,共4页Stomatology

摘　　要：目的检测磁珠分离细胞STRO-1+的DPSC、EMSC中HNK-1、Nestin的表达,进一步了解DPSC的表型特点及与EMSC的相关性,为今后研究提供较为纯化的细胞来源。方法采用间接免疫磁珠分离法获得DPSC、EMSC,间接免疫荧光双标法检测抗原HNK-1、Nestin的表达。结果磁珠分离前后进行细胞计数,大约有5%的DPSC为STRO-1+的细胞,大约有1%的EMSC为STRO-1+的细胞;STRO-1+的DPSC免疫荧光检测同时表达HNK-1(++)、Nestin(+),STRO-1+的EMSC免疫荧光检测显示也同时表达HNK-1(++)、Nestin(++)。结论免疫磁珠分离法获得STRO-1+阳性的DPSC共表达EMSC的标记HNK-1和Nestin,进一步说明二者作为间充质来源的干细胞,细胞表型具有继承性。Objective The expression of HNK-1 and Nestin will be checked in the STRO positive DPSC and EMSC which are separated by immunomagnetic bead. To investigate the phenotype characteristics of DPSC and the it s correlation with EMSC to find out the purified cell source for the future study. Methods DPSC and EMSC were obtained with the indirect immunomagnetic bead seperation method. The expression of HNK-1 and Nestin was checked with the indirect immunofluorescent double marker. Results Cell counting was made before and after immunomagnetic bead separation. About 5% DPSCs and 1% EMSCs were STRO-1 positive. HNK-1 was sim- ultaneously expressed in STRO-1 positive DPSC. HNK-1 and Nestin were simultaneously expressed in STRO-1 positive EMSC. Conclu- sions The STRO-1 positive DPSC expressed the EMSC marker of HNK-1 and Nestin,which shows that both of them are the stem cells from the mesenchymal tissue and the phenotype has the inheritance.

关 键 词：免疫磁珠分离 牙髓干细胞 外胚间充质干细胞 STRO-1 HNK-1 NESTIN 

分 类 号：R392.12[医药卫生—免疫学]