精索静脉曲张大鼠附睾组织中低氧诱导因子1α 和p53的表达  

Expressions of hypoxia inducible factor 1 α and P53 in the epididymis of varicocele rats

作　　者：梁明[1,2] 赵鲁刚[3] 史本康[2] 李景勤[4] 

机构地区：[1]山东中医药大学第二附属医院生殖医学科,山东济南250001 [2]山东大学齐鲁医院泌尿外科,山东济南250012 [3]中山大学附属第六医院生殖医学研究中心,广东广州510655 [4]解放军第401医院泌尿外科,山东青岛266071

出　　处：《中华男科学杂志》2014年第7期613-617,共5页National Journal of Andrology

基　　金：山东省自然科学基金(ZR2011HM013)~~

摘　　要：目的:观察精索静脉曲张(VC)大鼠附睾指数,附睾精子活率,附睾组织中低氧诱导因子1α(HIF-1α)、p53的表达及上皮组织的变化,探讨VC大鼠附睾功能降低的机制。方法:90只SD大鼠随机分为手术组、假手术组和正常对照组,每组30只。手术组建立SD大鼠左侧VC模型,采用假手术建立假手术组,正常对照组不作任何处理。各组大鼠均于手术组术后49 d断颈处死。处死前大鼠称重,处死后左侧附睾称重,收集附睾尾部精子,以计算机辅助精子分析系统行精子活率检测;应用Western印迹检测附睾组织中HIF-1α和p53的表达变化;HE染色观察附睾上皮组织的情况。结果:手术组27只大鼠建模成功。3组大鼠附睾指数差异无统计学意义[(40.53±1.76)×10-5、(43.31±1.58)×10-5、(44.10±2.62)×10-5,P>0.05];手术组大鼠精子活率[(71.86±5.07)%]和前向运动精子百分率[(42.26±4.45)%]均显著低于假手术组[(78.51±4.50)%、(49.08±4.19)%]和正常对照组[(79.24±2.70)%、(52.23±2.23)%](P均<0.05);手术组大鼠附睾组织HIF-1α和p53相对表达量[1.74±0.16、1.71±0.11]均显著高于假手术组[0.32±0.08、0.56±0.13]和正常对照组[0.12±0.03、0.25±0.06](P均<0.01);手术组附睾管上皮细胞与另外两组相比明显疏松无序并且数量减少。结论:VC导致附睾组织低氧并可能使附睾功能降低。Objective ： To investigate the mechanism of epididymal hypofunction of rats with varicocele （VC） by observing the changes in the epididymal index, motility of epididymal sperm, expressions of hypoxia-inducible factor 1 alpha （HIF-1α） and the tumor suppressor protein p53, and epididymal epithelial cells. Methods ： Ninety SD rats were equally randomized to a VC model （A）, a sham operation （B）, and a normal control group （C）. At 49 days after surgery, all the rats were executed after weighing. Then the volume of the left epididymis was obtained, the epididymal sperm motility was detected by computerassisted sperm analysis（CASA） , the expressions of HIF-1α and p53 in the epididymal tissue were determined by Western-blot, and the epididymal epithelial ceils were observed by HE staining. Results： VC models were successfully established in 27 of the rats. One-way ANOVA test showed no statistically significant differences in the epididymis index among groups A （ [40.53 ±1.76] x 10 -5 ）, B （ [43.31 ± 1.58] × 10 - 5 ）, and C （ [ 44.10 ± 2.62 ] × 10 - 5 ） （ p 〉 0.05 ）. Sperm motility and the percentage of progressively motile sperm were signifi- cantly lower in group A （ [71.86 ±5.07]% and [42.26±4.45]% ） than in B （ [78.51 ±4.50]% and [49.08 ±4. 191% ） and C （ [ 79.24 ± 2.70 ] % and [ 52.23 ± 2.23 ] % ） （both P 〈 0.05）, while the expressions of HTF-1α and p53 were remarkably higher in A （1.74±0.16 and 1.71 ±0.11） than inB （0.32±0.08 and0.56±0.13） and C （0.12±0.03 and0.25±0.06） （bothP〈 0.05 ）. The epididymal epithelial cells in group A were obviously decreased in number and arranged in loose and disorderly patterns as compared with those in B and C. Conclusion： Varicocele can cause hypoxia in the epididymal tissue, which in turn may lead to epi-didymal hypofunction.

关 键 词：精索静脉曲张 低氧诱导因子1Α P53 附睾 不育症 SD大鼠 

分 类 号：R697.24[医药卫生—泌尿科学]