望春玉兰种质资源遗传多样性ISSR分析  

Genetic Diversity of Magnolia biondii (Magnoliaceae) Assessed by ISSR Polymorphisms

作　　者：郭乐[1] 康永祥[1] 邢振杰 杨玲[1] 郭健[1] 李娜[1] 

机构地区：[1]西北农林科技大学林学院,杨凌712100

出　　处：《植物研究》2014年第4期465-473,共9页Bulletin of Botanical Research

基　　金：国家林业局公益性行业科研专项(200904004)

摘　　要：利用ISSR分子标记法对陕西、河南、湖北三省共35份具有代表性的单株野生望春玉兰种质进行遗传多样性和亲缘关系分析。从100条ISSR引物中筛选出来的10条多态性引物对供试材料进行PCR扩增后共获得154条条带，其中多态性条带143条（PPB=93．5％）；根据ISSR扩增结果，35份材料间的遗传相似系数（GS）为0．55～0．87；Nei’s遗传多样度（日）为0．2747；Shannon多态信息指数（I）为0．4274；不同地区间的遗传分化系数（e。）为0．1291。以上说明望春玉兰种质存在较丰富的遗传多样性，不同地区间和地区内均存在遗传分化，且地区内的变异对总变异的贡献相对较大。基于UPGMA法的聚类分析将35份供试材料分为四大类，其结果呈现出一定的地域性分布规律，与花色并无绝对关联。主成分分析结果基本支持聚类分析的结果。基于遗传距离和地理距离的Mantel检测显示，望春玉兰供试材料之间的遗传关系与它们的地理来源并无严格的一致性关系。The genetic diversity and relationship of 35 wild Magnolia biondii accessions （came from Shannxi, Henan and Hubei provinces ） were analyzed by using ISSR （Inter-simple sequence repeat ） markers. Ten primers selected from 100 ISSR primers （published by Columbia university） were used for amplification. A total of 154 bands were generated, of which 143 bands were polymorphic bands. The percentage of polymorphic loci （PPB） was 93.5%. According to the result of ISSR amplification, the genetic similarity coefficient among 35 M. biondii accessions ranged from 0.55 to 0.87; Nei＇ s gene diversity index （H） was 0. 274 7; Shannon diversity index （1） was 0. 427 4. The coefficient of gene differentiation （ Gst ） among 3 regions was 0. 129 1. All these results illustrated that there was an abundant genetic diversity in M. biondii species. A significant genetic differentiation existed both within and among different regions, moreover, the former was relatively large proportion of the total differentiation. The UPGMA dendrogram demonstrated that 35 germplasms were classified into 4 major groups based＂ on Nei＇ s similarity coefficient, which showed that there were some rules in geographical distribution, however, no absolute correlation with color. The result of principal coordinates analysis basically accorded with the UPGMA dendrogram analysis. Mantel test based on genetic distance and original geographic distance revealed that the genetic relationship among the studied germplasms was not strictly correlated with the geographical provenance.

关 键 词：望春玉兰 ISSR标记 遗传多样性 种质资源保护 

分 类 号：S685.15[农业科学—观赏园艺]