芍药组织培养中地下芽污染的克服  

Contamination Control of Underground Buds in Peony Tissue Culture

作　　者：曲文静[1] 李青[1] 刘燕[1] 

机构地区：[1]北京林业大学园林学院,国家花卉工程技术中心,北京100083

出　　处：《植物研究》2014年第4期524-528,共5页Bulletin of Botanical Research

基　　金：商品花卉设施生产关键技术优化升级研究与示范项目(2011BAD12B02-01)

摘　　要：针对芍药地下芽组织培养中污染严重的问题，对不同外植体的选择及不同消毒处理方法进行了研究。结果表明：芍药的地下芽培养应该选取生长健壮、饱满紧实的中等大小芽子；对于不同大小的地下芽应采取不同时长的HgCl2消毒处理，以5—8min为宜。对地下芽进行消毒处理时要剥去外面数层鳞片且至少保留2层鳞片；芍药地下芽以一步灭菌法为宜，太多的处理增加了其感染细菌的概率且无法保证灭菌彻底。The selection of different peony explants and sterilization methods were studied to suppress the tugla contamination rate in underground bud tissue culture of peony. The results show that healthy plump underground buds in moderate size should be first considered and different HgC12 sterilization time should be applied according to the buds＇ size, and 5 -8 rain is advisable. Striping several outer scale layers and leaving at least 2 inside scale layers and choosing the one-step sterilization method could reduce the contamination rate and ensure a thorough sterilization.

关 键 词：组织培养 芍药 污染 消毒处理 HgCl2 灭菌法 外植体 芽培养 

分 类 号：S682.12[农业科学—观赏园艺]