长爪沙鼠动情周期血清激素水平与卵泡发育的动态研究  

Dynamic study on levels of serum PRL,LH,FSH,E_2 and development of ovarian follicles in different phases of estrus cycle in gerbils

作　　者：肖丽[1] 陈立青[1] 戴方伟[1] 宋晓明[2] 郭红刚[1] 卢领群[1] 吕宇[1] 萨晓婴[1] 

机构地区：[1]浙江省医学科学院实验动物与安全性评价重点实验室,杭州310013 [2]杭州师范大学实验动物中心,杭州310036

出　　处：《中国比较医学杂志》2014年第6期30-34,I0002,共6页Chinese Journal of Comparative Medicine

基　　金：科技部"十二五"科技支撑计划项目子项目(2011BAI15B01-31;2013BAK11B01-41);省医药卫生平台研究计划重点项目(2012ZDA010);浙江省科技计划项目(2012C37097)

摘　　要：目的研究长爪沙鼠动情周期血清催乳素(PRL)、黄体生成素(LH)、促卵泡利激素(FSH)和雌二醇(E2)水平和卵泡黄体数量的变化。方法采用直接镜检法确定长爪沙鼠的动情周期,以ELISA法测定长爪沙鼠血清PRL、E2、FSH和LH水平,并观察动情周期各时相各级卵泡和黄体数量的变化情况。结果直接镜检法计数角化细胞统计阴道脱落细胞的类型及比例,确定长爪沙鼠动情周期的四个时相,发现长爪沙鼠动情周期各级卵泡数量各时相间均差异未见显著性。动情间期黄体数量较其他各时相显著减少(P<0.05)。血清PRL和LH水平从动情前期至动情间期逐渐增高,至动情间期达峰值(P<0.05),而血清E2和FSH水平均在动情前期最高(P<0.05),在动情间期达到最低。结论长爪沙鼠动情周期各时相卵泡和黄体数量与各时相激素的变化密切相关,动情周期各时相卵泡数量差异未见显著性。动情周期中出现的血清FSH和E2峰值的时相与小大鼠一致,而PRL和LH出现峰值的时相与大小鼠有差异,为长爪沙鼠生殖生理特性的进一步研究提供了参考。Objective To investigate the numbers of corpus luteum and ovarian follicles and compare the levels of serum prolactin（PRL）,luteinizing hormone（LH）,follicle-stimulating hormone（FSH） and estradiol（E2） in different phases of estrus cycle in female gerbils. Methods Consecutively taking vaginal smears of the gerbils and directly examined under light microscope to distinguish the four phases of the estrus cycle. Hematoxylin and eosin staining was used to histological examination of the gerbil ovaries,and to detect the levels of serum PRL,LH,FSH and E2 by ELISA assay during estrus cycle. Results The proportion of cornified vaginal exfolliated cells could be the basis to distinguish fourphases respectively： proestrus,oestrus,metoestrus,and dioestrus. Moreover,there were no significant differences between the numbers of ovarian follicles in different phases of estrus cycle. The numbers of corpus luteum in preoestrus were significantly lower than that in the other phases of estrus cycle（P ＆lt; 0. 05）. The levels of serum PRL and LH were increasing constantly from preoestrus to dioestrus,and both reached a peak at dioestrus（P ＆lt; 0. 05）. The levels of serum FSH and E2 both peaked at preoestrus,and were significantly higher than those at oestrus,metoestrus and dioestrus（P ＆lt;0. 05）. Conclusions There are no significant differences between the numbers of ovarian follicles in different phases of estrus cycle. Gonadotropin,prolactin and estradiol paly important roles in the regulation of estrous cycle. The phases during which surges of FSH and E2 occur in Mongolian gerbils are similar to those of rats and mice,while the PRL and LH are different. Our findings provide further reference to the study of reproductive physiology of Mongolian gerbils.

关 键 词：动情周期 长爪沙鼠 卵泡 催乳素 雌二醇 促卵泡刺激素 黄体生成素 

分 类 号：R33[医药卫生—人体生理学]