脐带间充质干细胞培养中的染色标记及示踪技术  

Umbilical cord-derived mesenchymal stem cell culture: dyeing and tracer technique

作　　者：黄霞[1,2] 潘兴华[1] 庞荣清[1] 阮光萍[1] 蔡学敏[1] 

机构地区：[1]解放军成都军区昆明总医院干细胞与组织器官工程研究中心,云南省干细胞工程实验室,云南省昆明市650032 [2]昆明医科大学临床学院,云南省昆明市650031

出　　处：《中国组织工程研究》2014年第23期3751-3755,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(31172170)~~

摘　　要：背景:脐带间充质干细胞的培养是进行脐带间充质干细胞研究时极其重要的部分,细胞培养技术的优化对推动间充质干细胞的临床应用特别是细胞治疗至关重要。同时,脐带间充质干细胞标记及示踪技术是干细胞移植治疗的研究热点之一。目的:对国内外脐带间充质干细胞染色标记技术的研究与进展作一综述。方法:以"干细胞,间充质干细胞,脐带间充质干细胞,细胞培养,染色标记"为中文捡索词,以"Umbilical cord-derived mesenchymalstem cells,Cell culture,Labeling methods"为英文检索词,检索维普和中国知网(CNKI)期刊全文数据库、Medline,highwire和外文生物医学期刊全文数据库(Foreign Journals Integration System)2001年1月至2013年10月有关脐带间充质干细胞的培养及标记染色文献。最终纳入35篇文献进行综述。结果与结论:脐带间充质干细胞尚未得到广泛应用,最关键的原因是脐带间充质干细胞的分离培养及染色技术不是很成熟,这些方面都值得在研究中进一步优化。虽然近几年脐带间充质干细胞标记及示踪技术有了较快的进展,但仍存在许多问题有待进一步解决。The culture of umbilical cord-derived mesenchymal stem cells is extremely important for studies on umbilical cord mesenchymal stem cells. Optimization of cellculture technology is crucial for clinical application of mesenchymal stem cells and even celltherapy. Meanwhile, the labeling and tracer technique of umbilical cord-derived mesenchymal stem cells is a hotspot in stem celltransplantation. OBJECTIVE：To review the research and development of the cellmarkers and tracer methods of umbilical cord-derived mesenchymal stem cells. METHODS：A computer-based search of VIP, CNKI, Medline, Highwire and Foreign Journals Integration System databases was performed for articles concerning culture and labeling of umbilical cord-derived mesenchymal stem cells published from January 2001 to October 2013. The keywords were“stem cells, mesenchymal stem cells, umbilical cord-derived mesenchymalstem cells, cellculture, labeling methods”in Chinese and English, respectively. Final y, 35 articles were included in result analysis. RESULTS AND CONCLUSION：Umbilical cord-derived mesenchymal stem cells have not yet been widely used, mainly because of the immature isolation, culture and staining techniques of umbilical cord-derived mesenchymal stem cells. These techniques are worthy of further optimization studies. Although in recent years, cellmarkers and tracer technology of umbilical cord-derived mesenchymal stem cell s have made great progress, there are stil many problems need to be solved.

关 键 词：干细胞 脐带脐血干细胞 间充质干细胞 脐带间充质干细胞 细胞培养 染色标记 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]