Effects of (–)-5-hydroxy-equol on the lifespan and stress resistance of Caenorhabditis elegans  

左旋-5-羟基-雌马酚对秀丽隐杆线虫寿命及抗性的影响(英文)

作　　者：张聪慧[1] 王秀伶[1] 梁晓琳[1,2] 张红蕾[1] 郝庆红[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071000 [2]华北制药集团,河北石家庄050000

出　　处：《Journal of Chinese Pharmaceutical Sciences》2014年第6期378-384,共7页中国药学（英文版）

基　　金：National Natural Science Foundation of China(Grant No.31170058);the Service Center for Experts and Scholars of Hebei Province(Grant No.CPRC027)

摘　　要：Caenorhabditis elegans （C. elegans） was used as an animal model to study the effect of （-）-5-hydroxy-equol, a microbialmetabolite of isoflavone genistein, on the lifespan, fecundity and resistance against thermal and oxidative stress. The resultsshowed that （-）-5-hydroxy-equol not only significantly increased the lifespan of C. elegans but also significantly enhancedthe resistance against thermal and oxidative stress at the concentrations of 0.1 mmol/L and 0.2 mmol/L. However, the fecundityof C. elegans was not obviously influenced after being exposed to the same concentrations of （-）-5-hydroxy-equol. Further studieson comparative transcriptome analyses and the lifespan ofdaf-16 （mu86） mutant and daf-2 （e1370） mutant indicated that（-）-5-hydroxy-equol prolonged the lifespan of C. elegans through DAF-2/DAF-16 Insulin/IGF-1 signaling pathway. This isthe first report that （-）-5-hydroxy-equol was able to increase the lifespan and improve the thermal and oxidative stress toleranceof C. elegans.以秀丽隐杆线虫模式生物为研究对象,研究大豆异黄酮染料木素微生物代谢产物左旋-5-羟基-雌马酚对线虫寿命、生殖能力以及抗热应激和抗氧化应激能力的影响。研究结果表明,浓度为0.1 mmol/L和0.2 mmol/L的左旋-5-羟基-雌马酚不仅能够显著延长线虫寿命,同时还能显著增强线虫耐热和抗氧化应激能力,而在相同浓度下线虫生殖能力则无明显不良影响。通过进一步对比分析转录物水平以及喂食相应线虫突变体daf-16(mu86)和daf-2(e1370),确定左旋-5-羟基-雌马酚延长线虫寿命与DAF-2/DAF-16 Insulin/IGF-1信号转导通路相关。有关左旋-5-羟基-雌马酚显著延长线虫寿命和增强线虫抗性为国内外首次报道。

关 键 词：（-）-5-Hydroxy-equol Caenorhabditis elegans LIFESPAN Thermal stress Oxidative stress FECUNDITY Insulin/IGF-1signaling pathway 

分 类 号：R962[医药卫生—药理学]