吸入乙酰半胱氨酸对博莱霉素导致的大鼠肺纤维化的影响  

The Effect of N-acetylcysteine in Mucolytic Treatment on the Bleomycin Induced Pulmonary Fibrosis in Rats

作　　者：赵帅[1] 李家宁[1] 丛莹莹[2] 陈国萍[3] 张艳玉[4] 

机构地区：[1]哈尔滨医科大学附属第二医院呼吸科二病房,黑龙江哈尔滨150086 [2]哈尔滨医科大学附属第三医院肿瘤内科,黑龙江哈尔滨150036 [3]天津市人民医院,天津300000 [4]哈尔滨医科大学附属第二医院,黑龙江哈尔滨150086

出　　处：《现代生物医学进展》2014年第25期4832-4836,共5页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅一般项目(11521096)

摘　　要：目的:研究N-乙酰半胱氨酸(NAC)雾化吸入对于博莱霉素(BLM)导致的大鼠肺纤维化的影响作用。方法:取清洁SD大鼠100只,平均分为10小组,1小组为对照组,其余每3小组为一组,分别为模型组,防治组,治疗组,通过在大鼠气管中注入BLM的方法来制造大鼠肺纤维化模型,防治组在造模前的7天以及造模后的7天内,治疗组在造模后的7天内,分别给予NAC雾化吸入治疗。在成功造模后的第7天、14天、28天,每组处死1小组大鼠,取出病理组织,通过免疫组化方法来观察肺组织纤维化的程度。研究NAC对大鼠肺纤维化的影响。结果:与模型组对比,防治组和治疗组在纤维化程度上明显较轻(P<0.05)。结论:吸入NAC对于博莱霉素导致的大鼠肺纤维化有明显的抑制作用,而且防治组疗效明显好于治疗组。既提前7天用药,效果更明显,大鼠获益更多。Objective： To study the projective effect of of N-acetylcysteine in mucolytic treatment on the bleomycin induced pulmonary fibrosis in rats. Methods： The pulmonary fibrosis was induced by intratracheal instillation of bleomycin in rats. 100 rats were involved and divided into 10 groups. One of the 10 groups was the normal control group, the other 9 groups were divided into the BLM group, the preventing group and the treatment group with 3 branches in each group. The rats in the preventing group were trated by the N-acetylcysteine for 7 days before molding. The rats in the treatment group were treated by tthe N-acetylcysteine for 7 days after molding. 10rats of each group were sacrificed after using ofbleomycin on days 7, 14, 28. The lungs were taken for histological study. The immunity histochemical method was taken to observe the fibrosis degree. Results： The degrees of pulmonary fibrosis in the preventing group and treating group were obviously reduced than the BLM group（P〈0.05）. Conclusion： The effect of N-acetylcysteine in mucolytic treatment on the bleomycin induced pulmonary fibrosis in rats was obviously,the curative effect in preventing group was better than treating group.Using N-acetylcysteine 7days before molding,rats benefit more.

关 键 词：N-乙酰半胱氨酸 大鼠肺纤维化 博莱霉素 

分 类 号：Q953[生物学—动物学] R563[医药卫生—呼吸系统]