Ets-1在姜黄素抑制心脏纤维化的表达研究  

Curcumin Through Reduced Ets-1 Expression Induced Inhibition of AngⅡHeart into Fibroblast Proliferation

作　　者：杨蕙宇[1] 潘瑾[1] 李平[1] 唐蕊[1] 向萌[1] 

机构地区：[1]西安医学院临床医学院内科教研室,陕西西安710021

出　　处：《现代生物医学进展》2014年第26期5045-5048,共4页Progress in Modern Biomedicine

摘　　要：目的:研究姜黄素的抗增殖作用是否依赖于其对Ets-1表达的下调。方法:使用Ets-1 siRNA对CFs细胞Ets-1基因进行沉默;Real-time PCR和western-blot法测定各组细胞Ets-1 mRNA和蛋白的表达水平。结果:在mRNA和蛋白水平上,AngⅡ明显增加CFs细胞内Ets-1的表达;使用siRNA技术对Ets-1进行沉默后,随着Ets-1表达的降低及转录调节能力的下降,由AngⅡ诱导的CFs细胞的增殖能力及增殖相关细胞因子分泌减少;姜黄素可以显著降低AngⅡ诱导的Ets-1表达升高,并具有浓度依赖性(P<0.05)。说明姜黄素对AngⅡ诱导的CFs增殖的抑制作用可能依赖于其对Ets-1表达的抑制作用。结论:Ets-1 siRNA对Ets-1进行基因沉默后,显示出抗增殖效用;姜黄素能够有效地抑制AngⅡ诱导的CFs细胞Ets-1 mRNA和蛋白的过表达;姜黄素的抗增殖作用可能依赖于其对Ets-1基因的表达下调而得以实现的。Objective： To observe the inhibition effect of curcumin （Cur） on AngⅡ-induced over-proliferation and overexpression of CTGF and PAI-1 in CFs and relation between its inhibitory effect and the suppressing expression of Ets-1. Methods： Ets-1 were measured by real-time PCR and western-blotting, respectively. After transfected with Ets-1 si-RNA, the cells were incubated with AngⅡ （100 nmol/L） for indicated times. The cell activity was detected by MTT assayand the levels of mRNA and protein of CTGF, PAI-1, Ets-1 were measured by real-time PCR and western-blotting, respectively. Results： With the lower expression of Ets-1, the proliferation which were induced by AngⅡ was decreased in CFs cells and the expression of CTGF and PAI-1 also declined. Curcumin can significantly reduced the expression of Ets-1 and the reduction was also concentration-related （P〈0.05）. Conclusion： Curcumin significantly inhibits AngⅡ-induced overexpression of Ets-1 in the levels of mRNA and protein in CFs, and Ets-1 siRNA also has the same inhibitory effect as Cur. These results suggested that Cur＇s anti-fibrotic effect, in part, depends on its inhibitory effect on the overexpression of Ets-1 in AngⅡ-stimulated CFs.

关 键 词：ETS-1 姜黄素 血管紧张素-Ⅱ 纤维化 

分 类 号：Q95-3[生物学—动物学] R542.23[医药卫生—心血管疾病]