人参皂苷Rb_1增生性瘢痕影响的研究  

Therapeutic Effect of Ginsenoside Rb_1on Hypertrophic Scar

作　　者：李昕珊[1] 岳毅刚[1] 张克勤 孙瑞军[3] 陈乃玲[3] 

机构地区：[1]桂林医学院附属医院整形外科,桂林541000 [2]桂林市南溪山医院整形外科,桂林541000 [3]河北联合大学,唐山063000

出　　处：《天然产物研究与开发》2014年第7期1014-1020,共7页Natural Product Research and Development

基　　金：广西壮族自治区卫生厅中医药科技专项项目(GZKZ1149)

摘　　要：本实验通过建立增生性瘢痕的动物模型探讨人参皂苷Rb1对增生性瘢痕的治疗效果。选取十分成熟的兔耳增生性瘢痕模型,每只兔子做5处增生性瘢痕,每个瘢痕做不同处理:空白对照组(a),生理盐水注射组(b),药物浓度分别为:0.2 mg/mL(c)、0.4 mg/mL(d)、1.0 mg/mL(e),共5组。通过HE、VG染色,计算HI和胶原纤维含量等发现人参皂苷Rb1可使得瘢痕增生情况有所抑制。免疫组织化学上相关胶原Ⅰ型蛋白和Caspase 3的表达以及原位杂交检测胶原Ⅰ型mRNA的表达从更深的角度表明了该药物有促进成纤维细胞凋亡的趋势和抑制胶原增生的现象。实验结果表明人参皂苷Rb1有抑制增生性瘢痕发展的作用。In this study, the therapeutic effect of ginsenoside RbI on hypertrophic scar was investigated through the establishment of animal model. A mature hypertrophic scar model on rabbits＇ ears was established and 5 hypertrophic scars were made on each rabbit. The hypertrophic scars were separated into 5 groups：blank controlgroup （a）, physiological saline group （b）, ginsenoside Rb, treatment groups ：0.2 mg/mL（c） ,0.4 mg/mL （d） and 1.0 mg/mL（e）. The results of HE, VG staining, HI and collagen fiber content showed that ginsenoside Rb1 can reduce the hyperplasia of scar. Furthermore, immunohistochemistry and situ hybridization were separately usedto test the expression of collagen I, Caspase 3 and mRNA of collagen I. It was found that ginsenoside Rbl can promote apoptosis of fibroblast and inhibit collagen hyperplasia. ttenee, it was concluded that ginsenoside Rb, played an effective role in the inhibition of hypertrophic sear development.

关 键 词：人参皂苷 增生性瘢痕 兔耳瘢痕模型 

分 类 号：R282[医药卫生—中药学]