海洋交替假单胞菌QD1-2抗肿瘤活性辅酶Q衍生物的分离与结构鉴定  

Isolation and Structure Elucidation of An Antitumor Ubiquinone Derivative from the Marine Bacterium Pseudoalteromonas rubra QD1-2

作　　者：陈晓兰[1] 丁立建[1] 何山[1] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,宁波315211

出　　处：《天然产物研究与开发》2014年第7期1021-1025,共5页Natural Product Research and Development

基　　金：浙江省海洋生物技术产业科技创新团队项目(2012R10029-02);宁波市海洋藻类资源高效开发利用创新团队项目(2011B81007);宁波大学引进人才启动基金项目(RCL2011718);宁波大学学科项目(XKL11D2103;XKC11002);宁波大学创新性开放实验建设项目(Cxxkf-201226)

摘　　要：海洋交替假单胞菌(Pseudoalteromonas rubra QD1-2)是从青岛海域分离筛选出的一株具有抗肿瘤活性的菌株。为了明确海洋交替假单胞菌(Pseudoalteromonas rubra QD1-2)的抗肿瘤活性成分和结构,利用大孔树脂柱层析、半制备HPLC等分离方法,首次从菌株QD1-2发酵液中分离到了一个单体化合物。通过高分辨率质谱、一维和二维核磁共振波谱等现代波谱技术进行结构鉴定,经测定,分子量为320,分子式为C18H24O5,为辅酶Q2衍生物。最后利用MTT法进行抗肿瘤活性测试,显示了较强的抗肿瘤活性。这一结果揭示了海洋交替假单胞菌产生新颖抗肿瘤化合物巨大的潜力,该属为寻找新型抗肿瘤天然化合物提供了新的来源。A marine bacterium,Pseudoalteromonas rubra QD1-2,was isolated from the Qingdao sea in China. The crude culture of QD1-2 was highly active against human tmnor cells. In order to confirm chemical structure of the anfitumor compound ,The crude metabolites of QD1-2 was isolated and purified by using column chromatography with Diaion HP20 maeroporous resin and then purified it by preparative HPLC. The chemical structure of the active compound finally ob- tained was achieved with spectroscopy methods ,including high resolution mass spectrometry andlD and 2D nuclear mag- netic resonance. According to the analysis results ,we identified the active compound as a ubiquinone derivative with the molecular weight of 320, the molecular formula C18H24O5. Finally antitumor activity was tested by the MTr method, showing very strong. The results revealed the huge potential of discovering novel antitumor compounds from Pseudoaltero- monas, providing new sources for antitumor natural compounds.

关 键 词：交替假单胞菌 结构鉴定 辅酶Q2 抗肿瘤活性 

分 类 号：R284.2[医药卫生—中药学]