HPLC同时测定药用白菊花中绿原酸、木犀草素、芹菜素和金合欢素  

Simultaneous Determination of Chlorogenic Acid,Luteolin,Apigenin and Acacetin in Medicinal Chrysanthemum morifolium by HPLC

作　　者：彭贵龙[1] 周光明[1] 秦红英[1] 

机构地区：[1]发光与实时分析教育部重点实验室,西南大学化学化工学院,重庆400715

出　　处：《天然产物研究与开发》2014年第7期1067-1071,共5页Natural Product Research and Development

基　　金：国家自然科学基金项目(21277110)

摘　　要：采用高效液相色谱法（HPLC）建立同时测定药用白菊花中绿原酸、木犀草素、芹菜素和金合欢素含量的方法，样品采用离子液体分散液相微萃法提取。采用PhenomenexC18色谱柱（250mill×4．6mm，5μm）；流动相：甲醇旬．2％冰醋酸溶液（65：35）；检测波长350nm；柱温35℃；流速0．8mL／min。绿原酸、木犀草素、芹菜素和金合欢素的质量浓度与峰面积分别在0．0005—100（r=0．9999）、0．00095—190（r=0．9999）、0．0008—160（r=0．9997）、0.00061—122μg／mL（r=0．9996）呈良好的线性关系；回收率分别为90．52％-100．44％、90．88％-98．58％、94．40％-98．82％、93．68％-100．54％。该方法快速，简便，重复性好，适合于同时测定药用白菊花中绿原酸、木犀草素、芹菜索和金合欢素的含量。A sensitive and specific high performance liquid chromatography （HPLC） method was developed to determine chlorogenic acid,luteolin,apigenin and acacetin in medicinal Chrysanthemum mw.follum. The C. morfolium sample was extracted by ionic liquid based dispersive liquid phase microextraction. The chromatographic separation was carried out on a Phenomenex C ls column （250 mm ×4.6 mm,5 μm） with methanol-0. 2% acetic acid （65：35, v： v） as mobile phases at a flow rate of 0.8 mL/min. The UV detection wavelength was set at 350 nm,and the column temperature was set at 35℃. The linear ranges were 0.0005 ～ 100 μg/mL for chlorogenic acid （r =0.9999） ,0.00095～ 190 μg/mL for luteolin （ r = 0. 9999）,0. 0008 ～ 160μg/mL for apigenin （r = 0. 9997 ） and 0.00061 ～ 122 μg/mL for acacetin （ r = 0. 9996） ,respectively;The recoveries of chlorogenic acid, luteolin, apigenin and acacetin were 90.52% ～100.44%, 90. 88%～ 98.58% ,94.40% ～98.82% ,93.68%～ 100.54%, respectively. The developed HPLC method was quick, simple and reproducible for the simultaneous determination of chlorogenic acid, luteolin, apigenin and acacetin in medici- nal Chrysanthemum morifolium.

关 键 词：药用白菊花 绿原酸 木犀草素 芹菜素 金合欢素 高效液相色谱法 

分 类 号：O652.62[理学—分析化学]