富硒灵芝调控大鼠乙酰辅酶A羧化酶α表达治疗非酒精性脂肪肝病的研究  

Effect of Se-enriched Ganoderma lucidum on Acetyl Coenzyme A Carboxylase αExpression of Rats in the Treatment of Non-alcoholic Fatty Liver Disease

作　　者：武俊紫 贾亚敏[3] 沈平瑞 胡跃高[5] 全胜麟[2] 李燕[2] 李树德[6] 

机构地区：[1]昆明理工大学昆华医学院,昆明650504 [2]云南省第一人民医院干部保健科,昆明650031 [3]太原理工大学现代科技学院,太原030021 [4]沈阳康硒生物工程研究所,沈阳110015 [5]中国农业大学农学与生物技术学院,北京100094 [6]昆明医科大学基础医学院,昆明650504

出　　处：《天然产物研究与开发》2014年第7期1086-1092,共7页Natural Product Research and Development

基　　金：国家自然科学基金项目(81360128);国家自然科学基金项目(81360252);云南省科技厅-昆明医科大学联合专项基金项目(2011FB225);云南省应用基础研究面上项目(2013FZ052);云南省应用基础研究面上项目(2013FZ183)

摘　　要：60只SD大鼠随机分为正常对照组、模型对照组、富硒灵芝治疗组。非酒精性脂肪肝病(Non-Alcoholic Fatty Liver Disease,NAFLD)模型建立成功后富硒灵芝治疗组给予富硒灵芝水灌胃治疗,模型对照组给予生理盐水灌胃辅助对比,空白对照组不加以任何干涉,分别于6周和12周处死大鼠各半。大鼠肝脏HE染色显示,治疗6周后富硒灵芝组脂肪变性程度及细胞肿胀相较于模型组明显减轻,炎性细胞浸润及坏死灶较模型对照组明显减少,富硒灵芝12周组大鼠比富硒灵芝6周组肝脏改善效果更佳;治疗6周后,富硒灵芝治疗组与模型对照组相比,血清中乳酸脱氢酶(LDH)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、谷胱甘肽(GSH)、丙二醛(MDA),肝脏组织中超氧化物歧化酶(SOD)、MDA和GSH,差异显著(P<0.05);SERBF-1mRNA和蛋白表达:富硒灵芝6周组与模型对照6周组相比,只有微小差异(P>0.05),12周富硒灵芝组与模型对照组相比,差距则具有统计学意义(P<0.05);ACCαmRNA和蛋白表达:富硒灵芝6周组与模型对照6周组相比,差别具有统计学意义(P<0.05);12周时差异更加显著;胰岛素抵抗指数6周时没有改善,12周时呈现出统计学意义(P<0.05)。因而推测,富硒灵芝可通过调控大鼠ACCα的表达、以提高抗氧化能力及改善肝功能来治疗NAFLD,但药效疗程较长,可能需长期服用。60 SD rats were randomly divided into normal control group, model control group, Se-enriched Ganoderma luci- dum treatment group. After non-alcoholic fatty liver disease （NAFLD） model was successfully established, the Se-en- rlched G. lucidurn treatment group received Se-enriched G. lucidum water gavage treatment and the model control group received saline auxiliary for comparison while the control group were treated with no interference. After 6 weeks and 12 weeks, half of the rats were sacrificed each time. HE staining showed that, after 6 weeks＇ treatment, the steatosis and cell swelling was significantly reduced in the rats with Seenriched G. lucidum treatment. The infiltration and necrosis in inflammatory cell was significantly reduced compared with the model group. The effect of 12 weeks＇ treatment with Se-enriched G. lucidum was better than that of 6 weeks＇. After 6 weeks ＇ treatment, the level of lactate dehydrogenase （LDH）, aspartate aminotransferase （AST）, alanine aminotransferase （ALT）, serum glutathione glutathione （GSH） ,malondialdehyde （MDA） ,liver superoxide dismutase （SOD） ,liver MDA and liver GSH in the group with Se- enriched G. lucidum treatment changed signiilcantly, compared with the control group, P 〈 0.05. Comparing the SERBF-1 mRNA and protein expression in Se-enriched G. luc/dum 6 weeks group with the expression in control group, there was only minor differences （P 〉 0.05） ,but the difference was statistically significance （P 〈0.05） between 12 weeks group and control group. ACCa mRNA and protein expression：comparing the Se-enriched G. luc/dwn 6 weeks＇ group with model control group, the difference was statistically significant （P 〈 0.05 ） and the difference was more significant at the 12 weeks＇ group. The 6 weeks＇ insulin resistance index （IR）, had no improvement, however,it was statistically slgniil- cant （P 〈 0.05） at the 12 weeks＇ group. Thus it was speculated that Se-enriched G. lucidum had the effect of regul

关 键 词：富硒灵芝 非酒精性脂肪肝 乙酰辅酶A羧化酶α 固醇调节元件结合转录因子1 

分 类 号：R285[医药卫生—中药学]