淫羊藿总黄酮的碱提工艺研究  

Extraction of Total Flavonoid from Herba Epimedii Maxim Using Alkali Solution

作　　者：李倩[1,2] 李晓[1,2] 魏悦[1,2] 范毅[1,2] 陈玲[1,2] 张海艳[1,2] 赵天增[1,2] 

机构地区：[1]河南省生物技术开发中心 [2]河南省植物天然产物开发工程技术研究中心,郑州450002

出　　处：《天然产物研究与开发》2014年第7期1141-1144,共4页Natural Product Research and Development

基　　金：河南省重大科技攻关项目(112101310600)

摘　　要：以淫羊藿苷和总黄酮的含量为指标,考察了提取溶剂及用量、提取时间、次数以及浓缩比例和酸用量对淫羊藿黄酮类成分提取的影响,确定了淫羊藿总黄酮碱提酸沉的最佳提取条件为:用10倍量的0.1%NaOH溶液加热煮沸2次,每次1 h,滤液浓缩至料液比1∶4后用盐酸调节pH为2,沉淀放置36 h后,真空过滤或离心分离干燥即得高含量提取物。该提取工艺提取率高,稳定性好,操作简便,适合工业化生产。A new method for the extraction of total flaconoid from Herba Epimedii Maxim was developed in this study. The extraction solvent, extraction duration, times of extraction, concentration ratio and acid dosage used for precipitation were investigated using the extraction yield of icariin and total flavonoid as considering indices. The extraction process was optimized as follows：using 10 times amount （compare to sample） of 0.1% NaOH solvent,boiling for 2 times and l h every time. The filtrate was concentrated to the ratio of 1： 4 for the ratio of solid to solvent. The pH of the concentrated filtrate was then adjusted to 2 by HCl followed by precipitation for 36 h and vacuum filtration or centrifugation of precipitate to afford flavonoid with high concentration. The developed method was stable, easy to operate and suitable for industrial production.

关 键 词：淫羊藿 总黄酮 碱提法 

分 类 号：R284.2[医药卫生—中药学]