舒芬太尼预处理对烫伤糖尿病和非糖尿病大鼠心肌损伤的影响  

Effects of sulfentanyl preconditioning on myocardial injury in scald in diabetic and non-diabetic rats

作　　者：吴财能[1] 屠伟峰[2] 马武华[1] 曹莹[3] 

机构地区：[1]广州中医药大学第一附属医院麻醉科,510450 [2]广州军区广州总医院麻醉科 [3]南方医科大学药学院新药评价中心

出　　处：《临床麻醉学杂志》2014年第7期701-704,共4页Journal of Clinical Anesthesiology

基　　金：广州市科技计划项目(2008Z1-E421)

摘　　要：目的观察舒芬太尼预处理对烫伤糖尿病和非糖尿病大鼠心肌损伤的影响。方法取糖尿病及非糖尿病SD大鼠各40只,随机分为八组(n=10):非糖尿病对照组(NS组)、非糖尿病烫伤组(NB组)、非糖尿病舒芬太尼预处理组(NP组)、非糖尿病纳洛酮拮抗组(NN组)及糖尿病对照组(DS组)、糖尿病烫伤组(DB组)、糖尿病舒芬太尼预处理组(DP组)、糖尿病纳洛酮拮抗组(DN组)。取烫伤后6h腹主动脉血,测血浆超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、TNF-α、心肌肌钙蛋白I(cTnI)水平,观察心肌组织形态学改变等。结果与NB组比较,NP组血浆SOD活性明显增加,TNF-α和cTnI浓度明显降低,心肌含水量明显减少(P<0.05);DB组TNF-α和cTnI浓度明显升高,心肌含水量增加(P<0.05)。与DB组比较,DP组血浆SOD活性明显增加,MDA、TNF-α和cTnI浓度明显降低,心肌含水量明显减少(P<0.05)。结论糖尿病可加重烫伤大鼠的心肌损害,舒芬太尼预处理可对其产生保护作用,其机制与减轻炎性反应和抗脂质过氧化损伤有关。Objective To explore effects of sulfentanyl preconditioning on myocardial injury in scald in diabetic and non-diabetic rats.Methods Eighty SD rats （40 diabetic rats and 40 non-diabetic rats）were divided into eight groups （10 rats per each）,including sham group（group NS,non-diabetic rats with sham burn）,burned group（group NB,non-diabetic rats with third-degree burns over 30%total body surface area （TBSA）and lactated Ringer＇s solution for resuscitation）,sulfentanyl group （group NP,non-diabetic rats without given sulfentanyl before burning and lactated Ringer＇s solution for resuscitation）and naloxone group（group NN,non-diabetic rats given naloxone before sulfentanyl group）,Diabetes sham group（group DS,diabetic rats with sham burn）,Diabetic rats burned group （group DB,diabetic rats given third-degree burns,over 30 percent of the total body surface area had been burned and given lactated Ringer＇s solution for resuscitation）,diabetic sulfentanyl group（group DP,diabetic rats given sulfentanyl before burning and given lactated Ringer＇s solution for resuscita-tion）and diabetic naloxone group（group DN,diabetic rats given naloxone,after that treated as the sulfentanyl group）.Results Compared to group NB,for the mice in group NP,the activity of plasma SOD increased significantly,TNF-α,cTnI and water content level in myocardium decreased signifi-cantly （P 〈0.05 ）;whereas TNF-α,cTnI and water content level in myocardium in group DB in-creased significantly （P 〈0.05）;Compared to group DB,for the mice in group DP,the activity of plasma SOD increased significantly,MDA,TNF-α,cTnI and water content level in myocardium de-creased significantly （P 〈0.05）.Conclusion Diabetes may deteriorate burn-induced myocardial injury in rats.Sulfentanyl pretreatment exhibits significant protective effects on burned-induced myocardial injury in severely burned diabetic rats via inhibiting lipid peroxidation and TNF-αexpression.

关 键 词：糖尿病 烫伤 脂质过氧化 肿瘤坏死因子-α 舒芬太尼 

分 类 号：R614[医药卫生—麻醉学]