乌司他丁剂量依赖性抑制紫杉醇诱导的触诱发痛  

Ulinastatin alleviates paclitexel-induced allodynia in a dose-dependent manner

作　　者：Diwas Manandhar 杜素娟[1] 杨涛[1] 叶西就[1] 

机构地区：[1]中山大学孙逸仙纪念医院南院区麻醉科,广州510289

出　　处：《岭南现代临床外科》2014年第4期417-420,共4页Lingnan Modern Clinics in Surgery

基　　金：国家自然基金项目(编号:30972857)

摘　　要：目的研究乌司他丁对紫杉醇引起周围神经病变的神经有保护作用。方法 24只SD大鼠随机分为3组,A组(生理盐水+紫杉醇组)、B组(乌司他丁25 kU/kg+紫杉醇组),C组(乌司他丁50 kU/kg+紫杉醇组)。各组大鼠分别连续7天(第1至7天)注射生理盐水或乌司他丁。动物于第1,3,5,7 d完成生理盐水或者乌司他丁注射后30 min腹腔注射紫杉醇2 mg/kg,即累计总剂量为8 mg/kg。分别于0,5,8,15,21,28天使用von Frey测痛仪测定大鼠机械刺激撤足阈值。结果 C组大鼠机械撤足阈值较A组和B组升高(P<0.01);而B组大鼠机械撤足阈值较A组升高(P<0.05)。结论乌司他丁能剂量依赖性地缓解紫杉醇诱导的触诱发痛,在周围神经病变中具有神经保护作用。Objective To evaluate the neuroprotective effect of ulinastatin in paclitexel-induced peripheral neuropathy. Methods Twenty-four SD rats were randomized into 3 groups, the rats in group A were administered normal saline and those in group B were administered 25 kU/kg of ulinastatin. Rats in group C were administered 50 kU/kg of ulinastatin. Normal saline or ulinastatin were i.p. once per day for 7 consecutive days （from days 1 to 7）. Paclitaxel （2 mg/kg per injection） was administered i.p. on four alternate days （Days 1, 3, 5 and 7; cumulative dose 8 mg/kg） after the injection of normal saline or ulinastatin. The paw withdraw threshold was measured using the yon Frey test on day 0, 5, 8, 15, 21 and 28 d. Results The paw withdrawal thresholds were significantly increased in rats of group C compared to the other groups （P〈0.01）, and the paw withdrawal thresholds in rats of group B were higher than those in group A （P〈0.05）. Conclusion Ulinastatin can alleviate paclitexelinduced allodynia in a dose-dependent manner, and it could have a neuroprotective effect in paclitexelinduced peripheral neuropathy.

关 键 词：紫杉醇 周围神经病变 乌司他丁 

分 类 号：R619[医药卫生—外科学]