一株海藻糖合成酶产生菌的鉴定及产酶条件优化  

Identification of a Pseudomonas sp. strain and optimization of the fermentation conditions of trehalose synthase

作　　者：奚悦[1] 高云[1] 卢小玲[1] 刘小宇[1] 

机构地区：[1]第二军医大学生物化学与分子生物学教研室,上海200433

出　　处：《中国微生态学杂志》2014年第7期751-754,共4页Chinese Journal of Microecology

基　　金：国家863项目子课题(2011AA09070302)

摘　　要：目的对一株海洋来源的产海藻糖合成酶菌株进行鉴定及产酶条件的初步优化。方法通过16S rDNA基因序列的同源性分析,对一株来源于东海海水的海藻糖合成酶产生菌进行鉴定,并通过单因素分析初步研究其培养特性和最佳的发酵条件。结果该菌16S rDNA序列与GenBank中已知序列相比,最高相似度为100%,鉴定为假单胞菌属(Pseudomonas),命名为Pseudomonas sp.A50。其最佳碳源和氮源分别为2%麦芽糖和0.5%酵母膏,最佳NaCl浓度为2.5%,在初始pH 7.8,接种量1%,装液量125 mL/250mL,28℃,130 r/min发酵48 h,海藻糖合成酶活力达到最高。结论此产海藻糖合成酶菌株为假单胞菌属,优化后,海藻糖合成酶活力达到14.16 U/mL。Objective To identify a trehalose synthase-producing strain isolated from ocean water and investigate the optimal composition of the medium and fermentation conditions. Methods 16S rDNA gene sequence was ana- lyzed for identification. The medium and fermentation parameters are obtained by single factor experiments. Results The new strain was named Pseudomoncts sp. A50. The optimal carbon and nitrogen sources were 1.5% maltose and 0.5% yeast powder, respectively. The optimal concentration of NaCl was 2.5%. The optimal liquid volume was 125 mL in 250 mL erlenmeyer; the inoculum was 1% , with the initial pH of 7.8. After cultivated for 48 hours in shaker at 28℃, 130 rpm, the maximum enzyme activity was obtained. Conclusion The new strain was classified as Pseudvmonas sp.. Under the optimal cultivation conditions, the maximum enzyme activity was up to 14.16 U/mL.

关 键 词：海洋假单胞菌 海藻糖合成酶 发酵培养基优化 

分 类 号：R378.991[医药卫生—病原生物学]