益生菌对感染小鼠早期IFN-γ、IL-4的影响  

Effects of probiotics on interfereon-gamma and interleukin-4 during the early stage of infection in mice

作　　者：夏嘉陵[1] 朱渝[2] 蒋虹[3] 万朝敏[2] 

机构地区：[1]攀枝花市妇幼保健院儿科,四川攀枝花617000 [2]四川大学华西第二医院儿科,四川成都610041 [3]四川省人民医院儿科,四川成都610072

出　　处：《中国微生态学杂志》2014年第7期794-797,801,共5页Chinese Journal of Microecology

基　　金：四川省卫生厅科研课题(20050013)

摘　　要：目的研究口服益生菌对鼠伤寒沙门菌(STM)感染小鼠Th细胞因子的影响,以探讨益生菌抗沙门菌感染的免疫学机制。方法将95只Balb/c小鼠分为4组,分别为益生菌组(P)、益生菌对照组(Pc)、正常感染组(I)和对照组(C)。P组口服益生菌,I组口服生理盐水,均予等剂量STM口服感染,Pc组接种益生菌但不感染STM,C组不作任何处理。各组动物在11个不同时点处死,观察小肠、肝脏、脾脏病理改变,ELISA测量血清IFN-γ、IL-4表达。结果益生菌组器官组织的病理改变轻微,IFN-γ较正常感染组明显增加(XP-I=66.52,P=0.001),且其表达在感染最初的1 h和后期的72 h分别出现两个高峰;IL-4明显降低(XP-I=-29.02,P<0.001),且较稳定。IFN-γ/IL-4比值扩大(XP-I=2.64,P<0.001)。结论口服益生菌使小鼠保持有利于抗STM感染的Th1型反应,减轻了STM对机体的免疫损伤。Objective To explore the effects of probiotics on the cytokine Th in Salmonella typhimurium （ STM ） - infected mice and discuss the immunological mechanism of the anti-STM infection role of probiotics. Methods 95 Balb/c mice were divided into probioties （P） group, problotics control （Pc） group, infection （I） group and control （C） group, respectively. The mice in group P were given orally probiotics, while the mice in group I were given orally saline; both groups were infected orally with S. thphimurium by equivalent dose. Mice in group Pc were o- rally given probiotics but not infected with STM. Mice in control group were untreated. All mice were sacrificed at a series of time points to observe pathological changes of spleen, liver and small intestine under a microscope. Serum intcrferon-γ （IFN-γ） and interleukin-4 （IL-4） were detected with ELISA. Results In probiotics group, the expression of IFN-γ was significantly higher than in infection group （Xp-1 = 66.52, P = 0.001 ） ; Two distinct expression peaks of IFN-γ appeared at 1 h and 72 h after inoculation respectively; Pathological lesion of mice was mild; The expression of IL-4 was distinctly lower with significantly difference than in infection group （Xp-1 =- 29.02, P 〈0.001 ） ; The ratio of IFN-γ/IL-4 was increased （Xp-1=2.64, P 〈0. 001 ）. The values of IFN-γ and IL-4 of probiotics control group and control group at all time points were not detectable. Conclusion Probiotics induced the expression level of serum IFN-γ, and decreased the expression level of serum IL-γ responding to STM infection in mice. Probiotics can promote immunological response against invaded pathogens and maintain suitable immunological response of organis.

关 键 词：益生菌 鼠伤寒沙门菌 细胞因子 干扰素-Γ 白细胞介素-4 

分 类 号：R378[医药卫生—病原生物学]