大鼠在高原习服过程中EPAS1基因表达的差异性研究  

Difference in EPAS1 Gene Expression of Rats Acclimatized to High Altitude Environments

作　　者：郝颖[1,2] 王荣[1,2] 谢华[1] 尹强[1] 贾正平[1,2] 李文斌[1] 王延玲[1] 鹿辉[1] 

机构地区：[1]兰州军区兰州总医院全军高原损伤防治重点实验室,甘肃兰州730050 [2]兰州大学药学院,甘肃兰州730000

出　　处：《解放军药学学报》2014年第3期185-187,共3页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家科技部重大资助项目;No.2008ZXJ09014-010;全军医药科研"十二五"重点项目;No.BWS12J012;全军医药科研"十二五"面上项目;No.CWS11C231

摘　　要：目的通过检测大鼠高原适应后各组织中EPAS1基因表达量的变化,为高原获得性习服提供分子机理研究基础及为急慢性高原病的发病机制进一步研究提供资料。方法通过实时PCR法检测平原组大鼠和高原适应(4350 m,7 d)组大鼠心、肝、脑、肺、肾组织中EPAS1基因的表达量。结果与平原组相比,大鼠在高原适应过程中各组织的EPAS1表达量均有升高,其中心、脑中表达量变化最大,分别为6.03倍和10.71倍。结论在高原适应性方面,各组织器官对适应性的贡献并不一致,为高原实验性研究提供依据。Objective To study the molecular mechanism of acquired altitude acclimatization and to shed light on the pathogenesis of acute and chronic plateau diseases by detecting the changes of EPAS1 gene expression in rats＇ organs. Methods The EPAS1 gene expression of rats＇ organs, including the heart,liver, brain, lung and kid- ney ,was determined in plain group（control） and in plateau（4350 m,7 d） group by real-time PCR. Results EPAS1 gene expression in different organs was higher in plateau group than in control group. The expression in the heart and brain in plateau group was 6.03 and 10.71 times higher than in control groups. Conclusion The contribution of different tissues and organs to altitude acclimatization is different.

关 键 词：高原适应 EPAS1基因 RT—PCR 基因表达 

分 类 号：R967[医药卫生—药理学]