益肺活血复方对模拟潜艇环境下家兔NO和ET-1的影响  

Effect of Yifei Huoxue Decoction on Secretion of NO and ET-1 of Rabbits Under Simulated Submarine Gas Environment

作　　者：张世超[1] 欧敏[2] 徐新宏[3] 许林军[3] 

机构地区：[1]南方医科大学第三临床医学院,广东广州510515 [2]海军总医院干部呼吸科,北京100048 [3]海军医学研究所舰艇卫生研究室,上海200433

出　　处：《解放军药学学报》2014年第3期213-215,共3页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：军队中医药科研专项课题;No.10ZYZ219

摘　　要：目的模拟潜艇环境,观察益肺活血复方对家兔血清和肺组织匀浆中一氧化氮(NO)及血管内皮素-1(ET-1)含量的影响。方法选用HOPE-MED 8050系列动式染毒系统模拟潜艇环境。家兔随机分为空白组、模型组、益肺活血复方低、高剂量(0.2、0.7 g·kg-1·d-1)组、地塞米松(0.2 ml·kg-1·d-1)组,每组15只,按照潜艇气体环境造模染毒,每天染毒4 h,同时每天肌肉注射给药1次,连续14 d后取家兔静脉血清及肺组织匀浆观察NO及ET-1水平变化。结果模型组较空白组血清及肺组织匀浆中NO含量显著升高(P<0.05),ET-1水平显著降低(P<0.05)。益肺活血复方高剂量组及地塞米松组与模型组相比较,血清、肺组织匀浆中NO含量均提高,ET-1水平降低,且均有统计学意义(P<0.05)。益肺活血复方高剂量较地塞米松提高NO含量效果更加明显(P<0.05)。益肺活血复方高剂量效果明显优于低剂量(P<0.05)。结论潜艇环境中部分有害气体长期作用会降低家兔肺组织及血液NO的含量,同时提高ET-1水平,益肺活血复方可以改善这两项指标,对家兔肺组织及血管起到一定的保护作用。Objective To observe the effect of Yifei Huoxue decoction on secretion of NO and ET-1 in serum and lungtissue of rabbits in a simulated submarine environment with harmful gases. Methods Main poisonous gases in submarines were mixed by HOPE-8050 series dynamic inhalation toxicology system. Seventy-five rabbits were random- ly and evenly divided into control group group, model group, Yifei Huoxue decoction low-dose （ 0.2 g 丶 kg- 1丶 d -1 ） group, Yifei Huoxue decoction high-dose（0.7 g丶kg-1丶d -1 ） group ,and dexamethasone（0.2 ml 丶kg-1 丶d -1 ） group. Af- ter 14 days in the exposure system,4 hours a day,the level of NO and ET-1 in rabbits＇ serum and lung tissue was ob- served. Results In model group,the NO content significantly reduced while the level of ET-1 increased（ P 〈 0.05 ）. In Yifei Huoxue decoction high-dose group and dexamethasone group, the content of NO in serum and lung tissue of rabbits was significantly higher than in control group. Conclusion The long-term effect of harmful gases in a subma- rine will reduce the NO content in lung tissue of rabbits, while increasing the level of ET-1. Yifei Huoxue decoction can improve the two indicators and effectively protect the rabbit lung tissue and blood vessels.

关 键 词：益肺活血复方 一氧化氮 内皮素-1 潜艇环境 

分 类 号：R965[医药卫生—药理学]