N-3多聚不饱和脂肪酸抑制犬无菌性心包炎模型炎症反应和心房颤动发生  

N-3 polyunsaturated fatty acids prevents atrial fibrillation and inhibits inflammation in a canine sterile pericarditis model

作　　者：张中[1] 郑强荪[2] 赵晋波[3] 刘力[2] 丁璐[2] 张超[2] 

机构地区：[1]济南军区青岛第一疗养院疗养二科,266071 [2]陕西西安第四军医大学唐都医院心血管内科 [3]陕西西安第四军医大学唐都医院胸外科

出　　处：《中华心律失常学杂志》2014年第3期220-225,共6页Chinese Journal of Cardiac Arrhythmias

摘　　要：目的 本研究采用犬无菌性心包炎模型来检验N-3多聚不饱和脂肪酸（PUFAs）对心房颤动（房颤）的影响及其对体内炎性因子、心房炎症状态的影响,对PUFAs的抗房颤作用提供新的证据,为房颤的治疗和预防提供新的思路.方法 20只犬随即分为对照组和PUFAs组,分别行开胸制作无菌性心包炎模型.PUFAs组实验开始前4周给予口服PUFAs（2 g/d）,术前和术后第2天分别测量心房各项指标（房颤的诱导性和持续性指标、心房有效不应期、房内传导时间）和C-反应蛋白（CRP）、白细胞介素-1β、白细胞介素-6、肿瘤坏死因子-α等水平.结果 术前两组间心房各项指标间差异无统计学意义,术后2 d PUFAs组较对照组CRP水平明显降低[（7.6±0.5）mg/dl对（11.7±1.3） mg/dl,P＜0.0001],IL-6水平明显降低[（202.9±23.8） pg/ml对（242.0±19.6） pg/ml,P＜0.001],IL-1β水平明显降低[（149.6±24.8）pg/ml对（233.5±24.8）pg/ml,P=0.0001],TNF-α水平明显降低[（83.3±8.5） pg/ml对（112.4±8.2）pg/ml,P＜0.0001],房颤可诱导性（11.0±7.4对28.0±10.3,P＜0.001）和持续性明显降低[房颤时程中位数：1105 s（655.8～ 1406.5 s）对2516.5 s（1187～3361 s）,P＜0.05],并且较对照组心房有效不应期（AERP）明显延长[（133.4±4.1）ms对（129.8±4.3）ms,P＜0.05],房内传导时间（CT）明显缩短[（46.6±4.4）ms对（51.9±4.8）ms,P＜0.05].结论 PUFAs可以明显降低术后房颤的发生危险,其对炎症反应的抑制作用可能是其机制所在之一.Objective It has been recently reported that atrial fibrillation （AF）is associated with tissue inflammation and inflammatory cytokines,and N-3 polyunsaturated fatty acids （PUFAs）have been reported anti-inflammatory effects in some epidemiological studies.However,the anti-inflammatory effect of PUFAs on AF is unclear.The purpose of the present study was to evaluate the anti-inflammatory effect of PUFAs on AF in a canine sterile pericarditis model.Methods Twenty dogs were randomly assigned to two groups：a control group （10 dogs）and a PUFAs treatment group （10 dogs）,in which sterile pericarditis was created by open-chest operation.PUFAs was administered orally（2 g/d）beginning 4 week before the operation until the end of the study.Before and 2 days after the operation,levels of CRP,IL-6,IL-1β,TNF-α,the inducibility and maintenance of AF,the atrial effective refractory period（AERP）,and intra-atrial conduction time（CT） were measured.Results Before the operation,there were no significant differences in any of the parameters between the two groups.On the second postoperative day,the PUFAs group had a lower CRP level [（7.6±0.5）mg/dl vs.（11.7± 1.3）] mg/dl,P＜0.0001,a lower IL-6 level[（202.9±23.8） pg/ml vs.（242.0±19.6） pg/ml,P＜0.0001],a lower IL-1β level[（149.6±24.8） pg/ml vs.（233.5±24.8）pg/ml,P=0.0001],a lower TNF-α level[（83.3±8.50 pg/ml vs.（112.4±8.2） pg/ml,P＜0.0001],a less AF inducibility（percentage of burst attempts leading to AF episodes：（11.0±7.4 vs.28.0± 10.3,P＜0.001） and maintenance [median AF duration：1105 s （655.8 ～ 1406.5 s） vs.2516.5 s（1187～3361 s）,P＜0.05],a longer AERP[（133.4±4.1） ms vs.（129.8±4.3） ms,P＜0.05],and a shorter intra-atrial conduction time [（46.6 ± 4.4） ms vs.（51.9 ± 4.8） ms,P ＜ 0.05] than that in the control group.Conclusion Dietary N-3 PUFAs supplementation attenuates the inducibility and maintenance of AF in an animal model of sterile pericarditis.Depression of proinfl

关 键 词：心房颤动 炎症 N-3多聚不饱和脂肪酸 

分 类 号：R541.75[医药卫生—心血管疾病]