羌活-独活合提液中总香豆素的大孔吸附树脂纯化工艺优选  

Optimization of Purification Process for Total Coumarins from Combined Extract of Notopterygii Rhizoma et Radix and Angelicae Pubescentis Radix by Macroporous Resin

作　　者：王蓉蓉[1] 杨素芳[1] 王英豪[1] 谢惠艳 邱颂平[1] 

机构地区：[1]福建中医药大学药学院,福州350108

出　　处：《中国实验方剂学杂志》2014年第15期25-27,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173573);福建省科技厅项目(2012D006)

摘　　要：目的:优选羌活-独活合提液中总香豆素的大孔吸附树脂纯化工艺条件。方法:采用UV测定总香豆素含量,检测波长300 nm。通过静态吸附-洗脱试验比较不同型号大孔树脂对总香豆素的分离纯化能力,以总香豆素含量为指标,利用单因素试验考察上样液质量浓度、上样量、洗脱剂体积分数及用量对羌活-独活合提液中总香豆素纯化工艺的影响。结果:D101型树脂对总香豆素具有较好的吸附和解吸附性能,最佳工艺条件为上样液质量浓度1.089 g·L-1,吸附流速1 BV·h-1,上样量2.5 BV,加4.5 BV水洗脱,弃去洗脱液,加80%乙醇2.5 BV洗脱,收集洗脱液;总香豆素吸附率、洗脱率及纯度分别为91.03%,84.80%,63.65%。结论:D101型树脂对羌活-独活合提液中总香豆素具有较好的分离纯化效果,优化的纯化工艺操作简单、稳定可行。Objective：To optimize purification process of total coumarins from combined extract of Notopterygii Rhizoma et Radix and Angelicae Pubescentis Radix by macroporous resin.Method：UV was employed to determine the content of total coumarins with detection wavelength at 300 nm.Types of resin for seperation and purification of total coumarins were screened by static adsorption-elution test,taking the content of total coumarins as index,single factor tests were adopted to optimize purification conditions with the concentration of sample solution,sample volume,eluent concentration and amount as factors.Result：D101 macroporous resin had adsorption and disadsorption ability for total coumarins,optimum purification conditions were as follows：the concentration of sample solution 1.089 g · L-1,adsorption flow rate of 1 BV ·h-1,sample volume of 2.5 BV,eluted with 4.5 BV of water and 2.5 BV of 80％ ethanol; adsorption rate,desorption rate and purity of total coumarins were 91.03％,84.80％ and 63.65％,respectively.Conclusion：Total coumarins from from combined extract of Notopterygii Rhizoma et Radix and Angelicae Pubescentis Radix could be purified effectively with D101 resin,this optimized process was simple,stable and feasible.

关 键 词：总香豆素 大孔吸附树脂 羌活 独活 静态吸附-洗脱试验 

分 类 号：R283.6[医药卫生—中药学]