不同栽培品种橘皮中主要活性成分的动态积累  

Dynamic Accumulation of Main Active Ingredient in Different Cultivars of Orange Peel

作　　者：刘荣[1] 韦正[1] 樊丹青[1] 杨丽[1] 陈鸿平[1] 刘友平[1] 

机构地区：[1]成都中医药大学药学院中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

出　　处：《中国实验方剂学杂志》2014年第15期111-117,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072991);四川省教育厅自然科学重点项目(09ZA033)

摘　　要：目的：比较不同采收时间3个栽培品种红橘、樟头红、南丰蜜橘皮中黄酮、生物碱、总酚酸、多糖类成分的含量,探讨橘皮类药材中上述4种活性成分及相对间的组成比例随采收时间的动态积累过程.方法：采用紫外-可见分光光度法测定橘皮中总黄酮、总酚酸、多糖的含量;HPLC测定药材中4种主要黄酮类成分、辛弗林的含量.HPLC测定药材中4种主要黄酮类成分及辛弗林含量,Hypersil BDS C18色谱柱（4.6mm×200 mm,5μm）,流动相为0.05％磷酸-乙腈梯度洗脱,柱温30℃,流速0.7 mL· min-,双波长检测283,335 nm,进样量5μL.结果：3种橘皮中黄酮、总分酸、辛弗林含量随采收时间的增加逐渐降低至果实成熟时趋于稳定,多糖则逐渐上升至成熟期达最高;红橘和樟头红中总黄酮与总分酸（7.01～11.14,6.37～9.14）的比例随果实成熟度增加而增加,而南丰蜜橘（6.25 ～4.04）则降低;3种橘皮中多糖与总分酸（6.18～21.45,2.70～4.40,6.36 ～ 14.51）的比例均随果实成熟度的增加而升高.结论：该研究为阐释我国传统中药青皮、陈皮两药“同源不同效、一体二用”的物质基础差异及橘皮类药材质量标准的升级提供参考.Objective：Compare three different cultivars of C.tangerina Hort.et Tanaka,C.reticulata Blanco cv.Kinokuni,Citrus reticulata Blanco cv.zhangshuensis Hort orange peel flavonoids,alkaloids,total phenolic acid,polysaccharides content with different harvesting time,exploring the orange peel herbs active ingredients in the above four main active ingredients and the relative composition ratio between harvest time with the dynamics accumulation.Method：Using ultraviolet spectrophotometry orange peel herbs total flavonoids,total phenolic acid and polysaccharides,HPLC determination of four main herbs flavonoids and synephrine content.Result：Three kinds of orange peel in flavonoids,total phenolic acid,synephrine content increased with the increase of harvest time gradually reduced to stabilize,polysaccharides are gradually increased to reach maximum maturity.The total flavonoids and total phenolic acids,polysaccharides and total phenolic acids between the ratio and the increases of fruit maturity are increasing.Conclusion：The study interpretation of traditional Chinese medicine peel,Citri Reticulatae Pericarpium and Citri Reticulatae Pericarpium Viride ‘homologous different effect and one material used as two kinds of Chinese medicine’ material differences and the quality standards of orange peel herbs further upgrade provide some reference.

关 键 词：橘皮 活性成分 黄酮 生物碱 总酚酸 多糖 动态积累 

分 类 号：R284.1[医药卫生—中药学]