肉苁蓉苯乙醇苷对大鼠高原肺水肿的防治作用  

Preventive Effect of Phenylethanoid Glycosides from Cistanches Herba on Rats with High Altitude Pulmonary Edema

作　　者：陶义存[1] 李建英[2] 许永华[2] 靳春丽[1] 屠鹏飞[3] 毛新民[1] 

机构地区：[1]新疆医科大学基础医学院,乌鲁木齐830011 [2]兰州军区乌鲁木齐总医院,乌鲁木齐830011 [3]北京大学药学院中医药现代研究中心,北京100083

出　　处：《中国实验方剂学杂志》2014年第15期134-138,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81160416)

摘　　要：目的:观察肉苁蓉苯乙醇苷预防给药对高原肺水肿模型大鼠的作用及可能的作用机制。方法:正常对照组,模型组,大花红景天口服液组(1.78 mL·kg-1·d-1),肉苁蓉苯乙醇苷低、中、高剂量组(75,150,300 mg·kg-1·d-1),每组12只,灌胃预防给药10 d,第8天起除空白对照组外其余各组置于模拟海拔5 000 m高原环境72 h建立高原肺水肿模型,观察各组大鼠肺组织病理改变,检测肺组织含水量、肺组织匀浆中白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)酶活力。结果:与正常对照组[肺组织含水量为(77.38±0.67)%,肺匀浆IL-6含量(181.49±26.00)ng·L-1,TNF-α含量(83.33±33.31)ng·L-1,MDA含量(5.25±1.13)nmol·mg-1,SOD酶活力(124.74±35.45)U·mg-1,GSH-Px酶活力(234.16±17.33)U·mg-1]相比,模型组大鼠出现明显的肺水肿,肺组织含水量显著升高、肺匀浆液中IL-6,TNF-α,MDA含量增高,SOD和GSH-Px酶活力显著降低。与模型组比较,肉苁蓉苯乙醇苷能够改善高原肺水肿大鼠肺水肿病理改变,降低肺组织含水量,降低肺匀浆中IL-6,TNF-α,MDA含量且提高SOD和GSH-Px的酶活力。结论:肉苁蓉苯乙醇苷能够预防高原肺水肿的发生,与其抗炎、抗氧化应激有关。Objective：To observe the effect and mechanism of phenylethanoid glycosides from Cistanches Herba on rat with high-altitude pulmonary edema.Method：Phenylethanoid glycosides from Cistanche Herba was given prophylactically to estibalish the rats model of high-altitude pulmonary edema by using hypobaric chamber in a simulated environment of 5 000 m altitude.The pathologic changes of rat lung tissue,the water content,the content of interleukin 6 （IL-6）,tumor necrosis factor-α （TNF-α）,malondialdehyde （MDA） and enzymatic activity of superoxide dismutase （SOD）,glutathione peroxidase （GSH-Px） in lung homogenate were measured.Result：Compared with the control group,the high altitude pulmonary edema model rats showed the remarkable character of pulmonary edema and the higher content of water in lung.The content of IL-6,TNF-α,MDA in lung homogenate were increased while enzymatic activity of SOD,GSH-Px were significantly decreased.The phenylethanoid glycosides from Cistanches Herba were able to improve pathological change of lung tissue,decrease water content,IL-6,TNF-α,MDA while increase enzymatic activity of SOD,GSH-Px in lung homogenate.Conclusion：Phenylethanoid glycosides from Cistanches Herba were able to prevent the high altitude pulmonary edema,the mechanism of which may related with the anti-inflammation,anti-oxidative stress in lung tissue.

关 键 词：肉苁蓉 苯乙醇苷 高原肺水肿 

分 类 号：R285.5[医药卫生—中药学]