复方蜥蜴散不同微粒组合剂对胃癌前病变模型大鼠Bcl-2和Survivin表达的影响  

Effects of Fufang Xiyi Powder Different Particles Combination Agent on Expression of Bcl-2 and Survivin in PLGC Model Rats

作　　者：李美丽[1] 朱西杰[1,2] 李卫强[1,2] 魏雪红[3] 蔡根深[1] 王延丽[1] 王儒[1] 

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学附属回医中医院,银川750004 [3]宁夏医科大学附属总医院,银川750004

出　　处：《中国实验方剂学杂志》2014年第15期150-153,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30020404)

摘　　要：目的:探讨复方蜥蜴散不同微粒组合剂(简称XY)创新模式(独特配方、不同微粒、糊剂给药)抗癌、阻癌及抑癌的分子生物学机制。方法:将120只SPF级SD大鼠随机抽取20只作为空白对照组,其余100只采用0.02 mol·L-1的甲基硝基亚硝基胍(MNNG)溶液按5 mL·kg-1灌胃等综合因素造模8周,成功制备胃癌前病变(precancerous leisions of gastric cancer,PLGC)大鼠模型后随机分为模型对照组、复方蜥蜴散80目组、100目组、80目100目等量混合组(XY80组、XY100组、XY80+100组均为1.5 g·kg-1·d-1)、维酶素组(0.3 g·kg-1·d-1)共5组,每组13只,均作相应处理后应用免疫组化法检测其对B淋巴细胞瘤/白血病-2(B-cell lymphoma/leuke-2基因,Bcl-2)和生存素(survivin)表达的影响。结果:各组大鼠胃黏膜组织Bcl-2和survivin的平均吸光度(A):空白对照组(19.06±2.32/21.46±2.69)、模型对照组(30.20±4.83/29.29±3.51)、XY80组(22.14±3.57/24.18±2.82)、XY100组(21.98±3.06/24.05±2.80)、XY80+100组(19.15±2.67/21.63±2.61)、维酶素组(24.80±2.89/26.59±3.36),经统计学分析,模型对照组与空白对照组比较,差异有统计学意义(P<0.05),各治疗组与模型对照组比较,差异有统计学意义(P<0.05),XY各治疗组与维酶素组比较,差异有统计学意义(P<0.05),尤以XY80+100组A最低,XY80组、XY100组与XY80+100组比较,差异有统计学意义(P<0.05);经Pearson相关分析,survivin与Bcl-2表达呈正相关(P<0.05),相关系数(r)=0.935。结论:Bcl-2和survivin在PLGC过程中具有协同作用;复方蜥蜴散不同微粒组合剂创新模式不仅配方独特,而且采用80目和100目等量混合和糊剂给药方式可进一步下调凋亡抑制基因Bcl-2和Survivin的表达,从而实现有效干预PLGC和延缓癌变进程的作用。Objective：The study was designed to explore the molecular biological mechanism of the innovation model （unique formula,different particles,paste medication way） that Fufang Xiyi powder different particles combination agent （XY）.Method：Twenty rats were as control group,the other rats were given 0.02 mol ·L-1 N-methyl-N＇-nitro-N-nitrosoguanidine （MNNG） to induce precancerous leisions of gastric cancer （PLGC） model,the modeling rats were randomly divided into 5 groups：model control group,80 mesh group,100 mesh group,80 mesh and 100 mesh mixing equal treatment group,Vitacoenzyme treatment group,13 rats each group.Munohisto chemical method was used to detect the B-cell lymphoma/leuke-2 （Bcl-2） and survivin protein expression of gastrictissue.Result：The average optical density （A） value of Bcl-2 and Survivin in gastric tissue in each group rats was：normal control group （19.06 ± 2.32/21.46 ± 2.69）,model control group （30.20 ± 4.83/29.29 ± 3.51）,80 mesh group （22.14 ±3.57/24.18 ±2.82）,100 mesh group （21.98 ±3.06/24.05 ±2.80）,80 mesh and 100 mesh mixing equal treatment group （19.15 ± 2.67/21.63 ± 2.61）,Vitacoenzyme treatment group （24.80 ±2.89/26.59 ± 3.36） model control group compared with normal control group was notable differences between them （P ＜ 0.05）.Each treatment group compared with model control group showed notable differences （P ＜ 0.05）,each treatment group of Fufang Xiyi powder compared with Vitacoenzyme treatment group was significantly lower （P＜0.05）,the A values of 80 mesh and 100 mesh mixing equal treatment group was the lowest,and that was significantly lower than 80 mesh group and 100 mesh group （P ＜ 0.05）.The expression of survivin protein was positively correlated with that of Bcl-2 protein （r =0.935,P ＜ 0.05）.Conclusion：The expression of Bcl-2 and Survivin proteins found in this study indicats a synergistic effect among PLGC.The innovation model of Fufang Xiyi powder different particles combinati

关 键 词：复方蜥蜴散 胃癌前病变 B淋巴细胞瘤 白血病-2 生存素 甲基硝基亚硝基胍 

分 类 号：R285.5[医药卫生—中药学]