清肺理痰方各有效成分组对脂多糖诱导的急性肺损伤大鼠的影响  

Effects of Effective Component Groups of Qingfei Litan Prescription on Acute Lung Injury Rats

作　　者：刘官斌[1] 潘俊辉[1] 王鹏[1] 李航[1] 

机构地区：[1]广州医科大学附属第一医院,广州510120

出　　处：《中国实验方剂学杂志》2014年第15期154-159,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广州市科技计划项目(2012J4300068)

摘　　要：目的:研究清肺理痰方各有效成分组对急性肺损伤大鼠的影响及可能的作用机制。方法:SD大鼠随机分为正常对照组、模型组、地塞米松组、清肺理痰方水提取物组、乙醇提取物组、石油醚萃取物组、乙酸乙酯萃取物组、正丁醇萃取物组、残余水层组;用以上中药复方各有效成分组预防性给药,采用气管内注入脂多糖致大鼠急性肺损伤;观察造模前后大鼠的一般情况;采用酶联免疫吸附试验(ELISA)检测血清中白介素(IL)-1β、肿瘤坏死因子(TNF)-α、白介素(IL)-6的水平;光镜下观察肺组织病理变化;免疫组织化学技术观察大鼠肺组织中核因子κB(NF-κB)p65蛋白表达情况。结果:与正常对照组比较,模型组大鼠肺组织呈现典型的炎症病理特征,肺组织损伤明显,肺湿/干重比、血清中IL-1β,TNF-α,IL-6含量及肺组织中NF-κB p65蛋白表达明显升高(P<0.01)。与模型组比较,清肺理痰方各有效成分组、地塞米松组肺组织病理损伤有不同程度地减轻,肺湿/干重比、血清中IL-1β,TNF-α,IL-6含量及肺组织中NF-κB p65蛋白表达出现不同程度的降低,其中以水提取物组和乙醇提取物组降低最明显(P<0.01或P<0.05),与地塞米松组疗效相当。结论:中药复方清肺理痰方各有效成分组可不同程度地减轻内毒素致急性肺损伤大鼠的病理损害,其机制可能通过抑制NF-κB信号转导通路,进而减少细胞因子(如IL-1β,TNF-α,IL-6)分泌以减轻脏器组织损伤,其中以水提取物组和乙醇提取物组疗效最明显。Objective：To study the effects of effective component groups of Qingfei Litan prescription on the acute lung injury in rats.Method：SD rats were randomly divided into nine groups：normal control group,model group,dexamethasone group,water extraction group,ethanol extraction group,petroleum ether extraction group,ethyl ether extraction group,n-butanol extraction group,residual water layer group.Acute lung injury model of rats was induced by intratracheal injection of lipopolysaccharide （LPS）.The general situation of the rats was observed.The enzyme-linked immunosorbent assay （ELISA） was used to detect interleukin （IL）-1β,tumor necrosis factor alpha （TNF-α）,interleukint （IL-6） levels in serum.The pathological changes of lung tissue was observed in light microscopy.Nuclear factor-kappa B （NF-κB） p65 protein expressions were measured by immunohistochemistry in lung tissue.Result：Compared with normal control group,the lung tissue showed typical pathological features of inflammation and damage evidently in model group.Lung wet/dry weight ratio,the levels of IL-1β,TNF-α,IL-6 in serum,and the expressions of NF-κB p65 protein was significantly higher （P ＜0.01）.Compared with the model group,effective component group of Qingfei Litan prescription and dexamethasone group show different degrees of mitigate trend in lung tissue damage,lung wet/dry weight ratio,the levels of IL-1β,TNF-α,IL-6 in serum,and the expressions of NF-κB p65 protein showed different degrees of mitigate trend,than water extraction group and ethanol extraction group had the most significant difference （P ＜ 0.01 or P ＜ 0.05）,and there was no significant difference compare with the dexamethasone group.Conclusion：The effective component groups of Qingfei Litan prescription can reduce pathological damage in the lung injury rats by LPS-induced.The mechanism may inhibit NF-κB protein signal transduction pathways,thereby reducing the secretion of cytokines （such as IL-1β,TNF-α,IL-6）.The water extracti

关 键 词：清肺理痰 脂多糖 急性肺损伤 细胞因子 核因子KAPPA B 

分 类 号：R285.5[医药卫生—中药学]