大豆异黄酮对去卵巢大鼠学习记忆和海马谷氨酸细胞及NR2B受体的影响  

Regulation of Glu Neuro-transmitter System and NR2B by Isoflovone in Ovariectomized Rats Brain

作　　者：买文丽[1,2] 刘行海[1,2] 刘亮[3] 董雯[4] 陈建林[5] 刘华[1,2] 

机构地区：[1]川北医学院生理教研室,四川南充637000 [2]川北医学院机能中心,四川南充637000 [3]川北医学院实验动物中心,四川南充637000 [4]北京中医药大学东方学院临床教研室,河北廊坊065000 [5]四川大学生命科学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《中国实验方剂学杂志》2014年第15期160-163,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省教育厅课题(09ZB121 11ZD159);川北医学院校级重点课题(CBY11-A-ZP17)

摘　　要：目的:探讨大豆异黄酮(isoflovone,IS)对去卵巢大鼠空间学习记忆和海马谷氨酸(Glu)细胞及谷氨酸受体2B(NMDAR2B)的影响。方法:选用青年SD雌性大鼠,去除双侧卵巢造模。随机分为6组,对照组、模型组、苯甲酸雌二醇(estradiol benzoate,EB)组、大豆异黄酮低、中、高(IS1,IS2,IS3,分别为20,40,80 mg·kg-1,ig)剂量组。EB组给苯甲酸雌二醇ip 0.2 mg·kg-1,每周1次,共6个月。观察各组大鼠Morris水迷宫学习能力,海马Glu神经元的表达及NR2B蛋白的表达。结果:与对照组比较,模型组定位航行和空间探索能力下降(P<0.05);EB组、IS3组定位航行学习能力和空间探索能力明显高于模型组(P<0.05)。与对照组比较,模型组海马Glu阳性细胞染色强度和NR2B蛋白表达均显著降低(P<0.05);与模型组比较,EB组和IS3组海马Glu阳性细胞染色强度和NR2B蛋白表达均显著增加(P<0.05)。结论:大豆异黄酮能提高去卵巢大鼠空间学习能力,机制可能与影响Glu含量,提高NR2B蛋白表达有关,并且大豆异黄酮的作用具有剂量依赖性。Objective：To explore the effects and mechanisms of isoflovone （IS） on spatial learning and memory and glutamate （Glu）,N-methyl-D-asprtate receptor 2B （NR2B） in ovariectomized rats brain.Method：The young femal Sprague-Dawley rats were ovariectomized.Ovariectomized rats were randomly divided into five groups,model group,estradiol benzoate （EB） group,IS low dose group （IS1）,IS middle dose group （IS2） and IS high dose group （IS3）.EB group were treated with estradiol benzoate （0.2 mg ·kg-1,once each week） by intraperitoneal injection once a week.IS groups were treated with Isoflovone （20,40,80 mg ·kg-1·d-1） by gavage once daily.The spatial learning and memory performance of all rats were evaluated by Morris watermaze task.The change of Glu was measured by immunohistochemistry.The levels of NR2B protein was measured by Western blotting.Result：Compared with control group,the latency period of model group increased apparently （P ＜ 0.05）.Compared with model group,the latency period of control group and IS3 group decreased apparently （P ＜0.05）.Compared with control group,the magnitude of tinction of glutamic acid in hippocampus and the protein levels of NR2B in model group decreased apparently （P ＜ 0.05）.Compared with model group,the magnitude of tinction of glutamic acid in hippocampus and the protein levels of NR2B in EB group,IS3 group increased apparently （P ＜ 0.05）.Conclusion：Isoflovone can promote the ovariectomized rats＇ impairment of spatial learning and memory by changing the contents of Glu and increasing the protein levels of NR2B in hippocampus.

关 键 词：大豆异黄酮 学习记忆 谷氨酸 N-甲基-D-天冬氨酸受体2B亚单位 

分 类 号：R285.5[医药卫生—中药学]