抗氧化中药有效组分复方的急性毒性及致突变性研究  

Research on Acute and Mutagenic Toxic Effects of Active Ingredients Assembling of Chinese Herbs for Antioxidant Activity

作　　者：杨鸿[1] 吴彦[2] 刘钊[1] 钟菊迎[1] 高尔宁 王萍娟[2] 黄东业[2] 田照福[2] 

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]广西中烟工业有限责任公司技术中心,南宁530001

出　　处：《中国实验方剂学杂志》2014年第15期185-189,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科学研究与技术开发计划课题(桂科攻11194005)

摘　　要：目的:根据前期中药组分配伍的抗氧化活性研究结果,采用甘草总黄酮、银杏叶提取物、淫羊藿苷、黄芪总苷和麦冬总皂苷组成中药有效组分最优配伍复方,研究该复方的急性毒性和致突变作用,为其进一步应用的安全性提供理论依据。方法:应用急性毒性试验、小鼠骨髓嗜多染红细胞微核试验、小鼠骨髓细胞染色体畸变试验和Ames试验检测该组分配伍复方的急性毒性和致突变性。结果:急性经口毒性试验,雌性、雄性小鼠经口最大耐受量(MTD)均>10 g·kg-1,属实际无毒。小鼠骨髓嗜多染红细胞微核试验、小鼠骨髓细胞染色体畸变试验和Ames试验结果均为阴性,显示在本实验条件下,该中药组分复方未见有致突变性作用。结论:中药组分配伍复方未见有明显的急性毒性和致突变作用,表明该药物安全性良好。Objective：To evaluate the acute and mutagenic toxic effects of the active ingredients assembling of Chinese herbs for antioxidant activity.Chinese herbal active ingredient formula was composed of total flavonoids of Glycyrrhiza,ginkgo biloba extract,total flavonoids of epimedium,astragaloside and total saponins in ophiopogonin.Method：Acute oral toxicity test,marrow cell micronucleus test polychromatic erythrocyte （PCE）,marrow chromosomal aberration test of mice and Ames were tested.Result：Mice acute oral maximum tolerated dose （MTD） of the formula was higher than 10 g ·kg-1.The micronucleus rates in all doses were not significantly different from the control group.Negative results were seen form chromosome aberration test.No increase in the number of revertant colonies was found in the Ames test with and without S9 activation system.Conclusion：The Chinese herbal active ingredient formula has no toxic substance and mutagenicity effects in this study.

关 键 词：中药有效组分配伍复方 急性毒性 微核 染色体畸变试验 AMES试验 

分 类 号：R285.5[医药卫生—中药学]