仁术健脾理气方对功能性消化不良大鼠胃排空功能及Ghrelin、5-HT、CGRP的影响  

Effect of Renzhujianpiliqi formula on the gastric emptying and Ghrelin,5-HT and CGRP in functional dyspepsia rats

作　　者：李晓玲[1,2] 张声生[2] 杨成[2] 汪正芳[2] 吴震宇[1,2] 储益平 熊鹰[2] 孙晓玮[1,2] 

机构地区：[1]北京中医药大学,北京100029 [2]首都医科大学附属北京中医医院,北京100010 [3]南京海思创新生物科技有限公司,南京210046

出　　处：《中国中西医结合消化杂志》2014年第7期355-359,共5页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：北京市科委课题--"仁术健脾理气颗粒临床前研究"(No:Z131100002513012);北京市卫生系统高层次卫生技术人才培养计划(No:2011-2-13)

摘　　要：[目的]研究仁术健脾理气方对碘乙酰胺(iodoacetamide,IA)诱导的功能性消化不良(FD)大鼠胃排空功能及胃促生长素(Ghrelin)、5-羟色胺(5-HT)、降钙素基因相关肽(CGRP)的影响,初步探讨仁术健脾理气方治疗FD的机制。[方法]雄性10日龄SD幼鼠100只,随机分为正常组(30只)和造模组(70只)。采用0.1%IA灌胃诱导大鼠FD模型。大鼠8周龄时进行模型评定,将造模成功的70只大鼠随机分为模型组(30只)、中药低剂量组(10只)、中药中剂量组(10只)、中药高剂量组(10只)和阳性药组(10只)5组。正常组与模型组予以0.9%氯化钠溶液1ml/100g灌胃,中药低、中、高剂量组分别给予生药含量0.477 5g/ml、0.955 5g/ml、1.911g/ml的中药煎剂1ml/100g灌胃,阳性药组给予0.3mg/ml多潘立酮溶液1ml/100g灌胃。采用苏木精-伊红染色观察大鼠胃组织病理变化。采用放射免疫法检测大鼠胃组织髓过氧化物酶(MPO)活性以及血清中Ghrelin、5-HT、CGRP的含量。[结果]胃排空结果显示,中药高剂量组和阳性药组大鼠3h摄食量和胃排空率显著高于模型组(P<0.05),而3h后胃内食物残余量显著低于模型组(P<0.05)。血清学结果显示,中药各剂量组和阳性药组FD大鼠血清中Ghrelin和5-HT的含量均不同程度地升高(均P<0.05),且高剂量组与阳性药组比较差异无统计学意义;中药各剂量组和阳性药组FD大鼠血清中CGRP的含量均不同程度地降低(均P<0.05),且高剂量组与阳性药组比较差异无统计学意义。[结论]仁术健脾理气方对IA诱导的FD大鼠具有加快胃排空、降低胃肠感觉过敏的作用,其机制可能与外周血Ghrelin、5-HT含量升高、CGRP含量降低有关。[Objective]To study the therapeutic effect of Renzhujianpiliqi formula （RF） on rats with functional dyspepsia（FD）,preliminary discuss its therapeutic mechanism.[Methods] One-hundred tenday-old male rats were randomly divided into control group（n＝30）and model group（n=70）.0.1％ iodoacetamide（IA）was used to induce FD rats.Model rats were evaluated at the age of 8 weeks and the successful model of the 70 rats were randomly divided into model group（n＝30）,low-dose of Chinese herbal medicine（CHM）group（n=10）,moderate-dose of CHM group（n=10）,high-dose of CHM（n=10）and domperidone group（n=10）.The control group and the model group were administered with 0.9％ Sodium Chloride Solution,CHM groups were orally administered RF for 7 days.Hematoxylin eosin（HE） staining was applied to observe the pathological changes of gastric tissue of the rats.Radioimmunoassay was used to detect the activity of MPO in gastric tissue and Ghrelin,5-HT,CGRP conten.[Results] Gastric emptying results showed：High-dose of CHM group and domperidone group rats consumed more solid food than the model group and presented a higher gastric emptying rate over 3-hour period（P＜0.05）,and the food in the stomach remnant was significantly lower than the model group after 3-hour period（P＜0.05）.CHM group and domperidone group increased the content of Ghrelin and 5-HT in serum of FD rats（bothP＜0.05） in different degrees,there was no significant difference between the high-dose of CHM group and domperidone group;CHM group and domperidone group decreased the content of CGRP in the serum of FD rats（P＜0.05） differently,there was no significant difference between the high-dose of CHM group and domperidone group.[Conclusion]RF can promote the gastric motility and reduce gastrointestinal hypersensitivity in FD rats induced by IA,and its mechanism might be related to the increase of the Ghrelin and 5-HT content and the decrease of CGRP content.

关 键 词：功能性消化不良 仁术健脾理气方 胃排空 胃促生长素 5-羟色胺 降钙素基因相关肽 

分 类 号：R256.3[医药卫生—中医内科学]