鼻咽组织荧光寿命影响因素研究  

A Study on the Factors Influencing Auto-fluorescence Lifetime of Nasopharyngeal Tissues In vitro

作　　者：陈明惠[1] 李昊[1] 陈荣[2] 宋成利[1] 

机构地区：[1]上海理工大学医疗器械与食品学院,教育部现代微创医疗器械及技术工程研究中心,上海200093 [2]医学光电科学与技术教育部重点实验室(福建师范大学),福建省光子技术重点实验室,福建福州350007

出　　处：《激光生物学报》2014年第3期207-210,共4页Acta Laser Biology Sinica

基　　金：国家自然科学基金(61308115);上海市自然科学基金(13ZR1457900);上海高校青年教师培养资助计划项目(zzslg12017);医学光电科学与技术教育部重点实验室开放课题(JYG1212)资助

摘　　要：本文在研究了离体人体鼻咽正常组织和癌变组织的荧光寿命的基础上,实验研究了生理盐水的浓度、组织光学特性参数、激发光源的偏振性对癌变和正常鼻咽组织的荧光寿命的影响。实验结果表明:组织的光学特性参数对组织的荧光寿命有不同程度的影响;而不同浓度的生理盐水和光源的偏振性对组织的荧光寿命没有显著的影响。荧光寿命与该发射荧光的强度没有关系,只决定于局部环境,受微环境的物理化学性质因素的影响,因此荧光寿命作为人体组织癌变的检测方法,有着很好的应用前景。The life-times of normal nasopharyngeal tissues and nasopharyngeal carcinoma tissues were determined.Fur-thermore,impact including concentration of brine,optical tissue properties,polarization of excitation light source on au-to-fluorescence lifetime of human nasopharyngeal carcinoma and normal nasopharyngeal tissues in vitro were investigated, respectively.The results show that optical tissue properties have different effects on fluorescence lifetimes of nasopharyn-geal tissues,while concentration of brine and polarization of excitation light source have no significant influence on fluo-rescence lifetime.Fluorescence lifetime is determined by physical and chemical properties of microenvironment and has nothing to do with fluorescence intensity,it can be used as a testing method for tumor.

关 键 词：光谱 自体荧光 荧光寿命 鼻咽组织 

分 类 号：Q631[生物学—生物物理学]