齐墩果酸对S180荷瘤小鼠细胞色素C和Caspase-3表达的影响  

Effect on Expression of Cytochrome C and Caspase-3 of S180 Tumor-bearing Mice Treated by Oleanolic Acid

作　　者：吴勃岩[1] 王雪[1] 董静[1] 刘文波[1] 孙阳[1] 郭艳莹[1] 王晓军[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药信息》2014年第4期14-16,共3页Information on Traditional Chinese Medicine

基　　金：黑龙江省教育厅科研项目(No.12531610);黑龙江省自然科学基金项目(No.D200844)

摘　　要：目的:探讨齐墩果酸诱导荷瘤小鼠肿瘤细胞凋亡作用机制。方法:将接种S180腹水瘤的小鼠随机分为模型组,齐墩果酸高、中、低剂量组,环磷酰胺组。采用免疫组化法检测肿瘤组织中细胞色素C和Caspase-3蛋白表达水平。结果:齐墩果酸对S180荷瘤小鼠细胞色素C和Caspase-3蛋白表达水平明显提高,与模型组比较差异显著(P<0.01),具有诱导肿瘤细胞凋亡作用。结论:齐墩果酸能上调S180荷瘤小鼠肿瘤细胞内细胞色素C和Caspase-3蛋白的表达,通过线粒体凋亡通路诱导肿瘤细胞凋亡而发挥抗肿瘤作用。Objective：The mechanism of cell apoptosis had been explored after tumor -bearing mice were trea-ted by oleanolic acid.Methods： There were five groups of S180 tumor -bearing mice, which were model group, oleanolic acid groups with different doses （ high, middle and low ） and cyclophosphamide group .The expressions of cytochrome C and Caspase -3 were measured by immunohistochemistry .Results： Oleanolic acid induced the protein level of cytochrome C and Caspase -3 increased compared with the model group （ P〈0.01 ） .Oleanolic acid may induce the tumor cell apoptosis .Conclusion：Oleanolic acid can up-regulate the protein expressions of cytochrome C and Caspase -3 of S180 tumor-bearing mice .It is through mitochondrial apoptotic pathway for exerting antitumor effect .

关 键 词：齐墩果酸 细胞凋亡 细胞色素C CASPASE-3 抗肿瘤 

分 类 号：R285.5[医药卫生—中药学]