二氢槲皮素对H_2O_2诱导H9c2心肌细胞损伤的保护作用  

Protective Effects of Dihydroquercetin against H_2O_2-induced Injury in H9c2 Cardiomyoblasts

作　　者：王知斌[1] 翟亚东 苏晓琳[1] 吕金南 于莹[1] 王秋红[1] 杨炳友[1] 匡海学[1] 

机构地区：[1]教育部北药基础与应用研究重点实验室,黑龙江省中药及天然药物药效物质基础研究重点实验室,黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药信息》2014年第4期16-19,共4页Information on Traditional Chinese Medicine

基　　金：黑龙江中医药大学杰出人才培育基金(No.2012jc02);高等学校博士学科点专项科研基金(No.20132327120002);黑龙江中医药大学"优秀创新人才支持计划"科研项目(No.2012RCD05)

摘　　要：目的:研究二氢槲皮素对H2O2诱导H9c2心肌细胞损伤的保护作用。方法:建立H2O2诱导H9c2心肌细胞损伤模型;利用四甲基偶氮唑蓝(MTT)法考察预孵育二氢槲皮素对H2O2诱导H9c2心肌细胞损伤细胞存活率的影响;利用试剂盒检测培养液中乳酸脱氢酶(LDH),细胞内丙二醛(MDA),过氧化氢酶(CAT)、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)含量的变化。结果:不同浓度的二氢槲皮素预处理4、12、24h均能够提高细胞的存活率,且呈剂量依赖性,其中预处理12h的保护作用最为明显。与正常对照组相比,模型组的存活率下降,LDH的漏出量增大,细胞内MDA的含量增大,CAT、SOD、GSH-Px的活力明显降低。用二氢槲皮素预处理12h能显著地减少LDH的漏出量,降低细胞内MDA的含量,提升细胞内CAT、SOD、GSH-Px的活力,并呈剂量依赖性。结论:二氢槲皮素对H2O2诱导H9c2心肌细胞损伤具有显著保护作用,且呈剂量依赖性,其作用机制可能与其能够提高细胞内抗氧化酶活性,增强细胞稳定性有关。Objective：To investigate the protective effects of dihydroquercetin against H 2 O2 -induced injury in H9c2 cardiomyoblasts.Methods：A model of H2O2 -induced injury in H9c2 cardiomyoblasts was established. Cell viability was determined colorimetrically by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazo-lium （MTT） assay.The supernates and cells were collected , respectively, after the different treatments for measuring the LDH and MDA levels, as well as SOD,CAT, and GSH-Px activity with the corresponding de-tection kit according to the manufacturer ’ s instructions .Results：H9 c2 cells were pretreated with dihydroquer-cetin for 12 h before exposure to 150 μM for 6 h.H2 O2 treatment caused severe injury to H9c2 cardiomyo-blasts.Dihydroquercetin pretreatment could attenuate H 2 O2 -induced injury , decrease the levels of LDH leakage and MDA, and increase the activities of SOD, CAT, and GSH-Px with a dose-dependent.Conclu-sion：Dihydroquercetin showed significantly protective effects against H 2 O2 -induced injury in H9c2 cardio-myoblasts in dose-dependent .The mechanism of this may be due to its increasing the activities of endogenous antioxidant enzymes , as well as improving the stabilization of myocardial cells .

关 键 词：二氢槲皮素 H2 O2 H9C2心肌细胞 抗氧化 活性氧自由基 

分 类 号：R285.5[医药卫生—中药学]