不同电针对慢性应激抑郁模型大鼠海马及血清细胞因子IL-1β、IL-6表达的影响  

Effect on the Expression of IL-1β,IL-6 in Hippocampus and Serum of Depressive Rats with Chronic Unpredictable Mild Stress Treated by Different Electro-acupuncture Treatment

作　　者：莫雨平[1] 邓晓丰[1] 纪倩[1] 滕金艳[1] 姚海江[1] 宋洪涛[1] 唐银杉[1] 许安萍[1] 张淑静[1] 李志刚[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中医药信息》2014年第4期107-111,共5页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.81072860)

摘　　要：目的:观察不同电针对慢性应激抑郁模型(chronic unpredictable mild stress,CUMS)大鼠海马及血清中细胞因子IL-1β、IL-6表达的影响,探讨IL-1β、IL-6在大鼠实验性抑郁症发病过程中的作用机制及不同电针干预对其影响。方法:将雄性清洁健康级SD大鼠60只,随机分为正常组、模型组、电针组、音乐电针组及百忧解组,每组12只。孤养结合CUMS 21天复制抑郁症模型,通过开野试验观察大鼠行为学的变化,采用免疫组化染色、酶联免疫吸附法分别检测IL-1β、IL-6在大鼠海马及血清中的表达情况。结果:模型组大鼠开野实验水平运动及垂直运动明显减少,电针组、音乐电针组及百忧解组均可改善此情况;模型组大鼠血清及海马内IL-1β、IL-6表达均明显升高,电针组、音乐电针组及百忧解组可逆转此病理变化,但三者之间无显著性差异。结论:抑郁大鼠的IL-1β、IL-6含量高于正常组;电针治疗抑郁症可能是通过降低IL-1β、IL-6的含量发挥抗抑郁作用。音乐电针与普通电针治疗抑郁症效果差异不明显。Objective：To investigate the effect on the expression of IL -1β, IL-6 in the hippocampus and ser-um of rats with chronic unpredictable mild stress treated by different electro -acupuncture（EA） treatment, to elucidate the possible role of IL -1β, IL-6 in the development of experimental depression and the effect of different EA for them .Methods：Adult male SD rats were randomly divided into normal control group （ n=12 ） , model group （ n=12 ） , pulsed EA group （ n=12 ） , music EA group （ n=12 ） and fluoxetine group （ n=12 ） . Depressive models were established by lonely raising and chronic unpredictable mild stress for 21 days. Open-field test was performed to test the behavior of rats .The expression of hippocampus and serum IL -1β, IL-6 was detected by using immunohistochemistry and enzyme linked immunosorbent assay （ ELISA ） techniques respectively .Results：The cross and rear score of model group was significantly lower than that in normal control group , while pulsed EA group , music EA group and fluoxetine group could reverse it .The ex-pression of hippocampus and serum IL -1β, IL-6 in model group increased significantly .The three kinds of treatment could improve these pathological change , but there was no difference among these effects of treat-ment .Conclusion：The levels of IL-1β, IL-6 in hippocampus and serum of depressive rats are higher than those in the normal group .EA treatment may have antagonism against depression by lowering the levels of IL-1β, IL-6 in hippocampus and serum ,and there is no difference between the effects of pulsed EA treat-ment and music EA treatment for depression .

关 键 词：电针 慢性应激 抑郁症 大鼠 海马 血清 细胞因子 

分 类 号：R245[医药卫生—针灸推拿学]