肝移植并FK506联合用药后人血清蛋白质组的二维凝胶电泳分析  

Two-dimensional gel electrophoresis analysis of human serum proteome after liver transplantation and FK506 combination therapy

作　　者：李彦[1] 王鸿丽[2] 赵永强[2] 刘煜[3] 梁艳[1] 

机构地区：[1]武警总医院药剂科,北京100039 [2]国家生物医学分析中心,北京100850 [3]武警总医院肝脏移植研究所,北京100039

出　　处：《武警医学》2014年第7期672-674,677,共4页Medical Journal of the Chinese People's Armed Police Force

基　　金：武警总医院二类课题(WZ2011019)

摘　　要：目的探索肝脏移植并FK506联合用药后人血清中蛋白质组的变化,研究免疫排斥反应的机制及FK506联合用药的作用。方法取5例肝癌患者移植前和移植后24 h、10 d、30 d、90 d、180 d的血清样品,用Aurum Serum Protein Mini Kit除高峰度蛋白,进行双向电泳(2-DE)分离,ImageMaster 2D Platinum图像分析软件分析电泳图谱。结果建立移植前和移植后并FK506联合用药的人血清双向电泳差异图谱,蛋白斑点数在(877±59)^(897±61),移植前、后不同组之间的匹配均>70%。共筛选出重复性好且表达显著差异的蛋白质斑点7个。结论初步建立了肝移植并FK506联合用药后人血清蛋白质组的2-DE分析方法。Objective To analyze the serum proteome change after liver transplantation and FK 506 combination in human ser-um by two-dimensional gel electrophoresis （2-DE） and to study the mechanism of immune rejection and the mechanism of action of FK506 combination.Methods The serum samples of 5 liver transplantation patients pre-and post transplantation at 24 hours,10days,30 days,90 days 180 days were treated using Aurum Serum Protein Mini Kit , and than analyzed by 2-DE.The electrophoresis imageswere analyzed by using ImageMaster 2D platirum imaging analysis software.Results The differential maps of 2-DE of serum proteomebefore and after liver transplantation and FK506 combination were established.Protein spots between （877 ±59.65）-（897.6 ±61.01）,the matching rate before transplantation and different groups after transplantation 〉70%.Among them 7 protein spots were selectedwith good repeatability and significant differential expression .Conclusions The 2-DE analytical method of serum proteome afterliver transplantation and FK506 combination was preliminarily established .The differential expression protein spots found in the studyhave laid a foundation for the further study .

关 键 词：肝移植 免疫排斥 免疫抑制药 FK506 血清 双向电泳 

分 类 号：R617[医药卫生—外科学]