紫花苜蓿MsARGOS基因的克隆及对拟南芥的转化  

Cloning of MsARGOS Gene from Alfalfa(Medicago sativa L.) and Transformation of Arabidopsis thaliana(L.) Heynh.

作　　者：王梦颖[1] 晁跃辉[1] 丛丽丽[1] 杨青川[1] 康俊梅[1] 张铁军[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国草地学报》2014年第4期52-59,共8页Chinese Journal of Grassland

基　　金："十二五"国家科技支撑计划课题(2011BAD17B01-01-3);中国农业科学院北京畜牧兽医研究所基本科研业务费项目(2014ywf-zd-2);现代农业牧草产业体系岗位科学家(CARS-35-04)

摘　　要：利用RT-PCR技术在紫花苜蓿中克隆出拟南芥ARGOS同源基因,命名为MsARGOS,MsARGOS基因编码区长234bp,编码77个氨基酸。通过DNA重组技术将其与载体pBI121连接,成功构建了植物表达载体35Spro::MsARGOS,并采用花序侵染法对拟南芥的腋生花序进行侵染,获得具有卡纳抗性的转基因植株,通过PCR和RT-PCR技术检测证明,目的基因已整合入拟南芥基因组中并能够成功表达。Based on the sequence of ARGOS gene from Arabidopsis thaliana （L.） Heynh, the homolo- gous gene was cloned from alfalfa, which was named as MsARGOS. The coding domain sequence of MsARGOS gene was 234bp in length, encoding 77 amino acids. By DNA recombination technology, MsARGOS gene was inserted into plasmid pBI121, resulting a plant expression vector 35Spro ：： MsARGOS. Transgenic Arabidopsis thaliana plants with kanamycin resistance were obtained by floral dip method with Agrobacterium tumefaciens harboring 35Spro：：MsARGOS. By PCR and RT-PCR analysis, the results showed that the target gene was inserted into genomes of Arabidopsis, and expressed in transgenic plants successfully.

关 键 词：紫花苜蓿 MsARGOS基因 拟南芥 转化 

分 类 号：S541.1[农业科学—作物学] Q943.2[生物学—植物学]